Changes in nitric oxide levels and their relationship with callose deposition during the interaction between soybean and Soybean mosaic virus

Changes in nitric oxide levels and their relationship with callose deposition during the... The present study aimed to investigate changes in nitric oxide (NO) level and its relationship with callose deposition during the interaction between soybean and Soybean mosaic virus (SMV). Soybean cv. ‘Jidou 7’ and SMV strains N3 and SC‐8 were used to constitute incompatible and compatible combinations. Intracellular NO was labelled with the NO‐specific fluorescence probe DAF‐FM DA. Confocal laser scanning microscopy (CLSM) was then used to observe changes in NO production during SMV infection‐induced defence responses in soybean. The results showed NO fluorescence increased rapidly at 2–72 h post‐inoculation, peaked at 72 h and then decreased in the incompatible combination. However, in the compatible combination, extremely weak NO fluorescence appeared in the early stage (2–24 h) post‐inoculation, but was not observed thereafter. Injections of the NO scavenger c‐PTIO prior to inoculation postponed the onset of NO production to 48 or 72 h post‐inoculation. The same occurred when injections of NR or NOS inhibitors were applied prior to inoculation. The observation of callose fluorescence in the incompatible combination revealed that either the elimination or reduction of NO in the early stage led to a delay in callose formation, enabling the virus to cause systemic infection. Together with our previous findings, this study indicates that viral infection could induce NO production and callose deposition during the incompatible interaction between soybean and SMV. The production of NO involves NR and NOS enzymatic pathways, and NO mediates the process of callose deposition at plasmodesmata. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Biology Wiley

Changes in nitric oxide levels and their relationship with callose deposition during the interaction between soybean and Soybean mosaic virus

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Publisher
Wiley
Copyright
© 2018 German Botanical Society and Royal Botanical Society of the Netherlands
ISSN
1435-8603
eISSN
1438-8677
D.O.I.
10.1111/plb.12663
Publisher site
See Article on Publisher Site

Abstract

The present study aimed to investigate changes in nitric oxide (NO) level and its relationship with callose deposition during the interaction between soybean and Soybean mosaic virus (SMV). Soybean cv. ‘Jidou 7’ and SMV strains N3 and SC‐8 were used to constitute incompatible and compatible combinations. Intracellular NO was labelled with the NO‐specific fluorescence probe DAF‐FM DA. Confocal laser scanning microscopy (CLSM) was then used to observe changes in NO production during SMV infection‐induced defence responses in soybean. The results showed NO fluorescence increased rapidly at 2–72 h post‐inoculation, peaked at 72 h and then decreased in the incompatible combination. However, in the compatible combination, extremely weak NO fluorescence appeared in the early stage (2–24 h) post‐inoculation, but was not observed thereafter. Injections of the NO scavenger c‐PTIO prior to inoculation postponed the onset of NO production to 48 or 72 h post‐inoculation. The same occurred when injections of NR or NOS inhibitors were applied prior to inoculation. The observation of callose fluorescence in the incompatible combination revealed that either the elimination or reduction of NO in the early stage led to a delay in callose formation, enabling the virus to cause systemic infection. Together with our previous findings, this study indicates that viral infection could induce NO production and callose deposition during the incompatible interaction between soybean and SMV. The production of NO involves NR and NOS enzymatic pathways, and NO mediates the process of callose deposition at plasmodesmata.

Journal

Plant BiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

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