cGMP‐Producing, Atrial Natriuretic Factor‐Responding Cells in the Rat Brain

cGMP‐Producing, Atrial Natriuretic Factor‐Responding Cells in the Rat Brain Using an in vitro incubation method, we stimulated cGMP production in rat brain slices by rat ANF‐(103–126). The localization of the cells responding to this ANF stimulation with an increase in cGMP production was studied by cGMP immunocytochemistry. ANF‐responding cells were found in specific loci throughout the central nervous system of the rat. Regions containing the highest number of these cells were: the olfactory bulb, the lateral septum, the bed nucleus of the accessory olfactory tract, the mediobasal amygdala, the central grey area, the medial vestibular nucleus, and the nucleus of the solitary tract. Scattered ANF‐responding, cGMP‐immunoreactive cells were found in the hippocampus, the cingulate cortex, the ventral pallidum, the medial preoptic area, and the endopeduncular nucleus. ANF‐responding cells in these areas had the same morphology, that is, multipolar with numerous processes. The nature of these ANF‐responding cells was studied by sequential staining with an antiserum against glial fibrillary acidic protein (GFAP). In the hippocampus it was demonstrated that all ANF‐responding cells are astroglial cells. However, not all astroglial cells in this area showed a cGMP response, demonstrating a regional heterogeneity. ANF‐responding cells, having the appearance of neuronal cell bodies, could be found in the subfornical organ, and the hypothalamic paraventricular nucleus. Fibres producing cGMP immunoreactivity in response to ANF were found in the median preoptic nucleus, the medial preoptic area, and the dorsal hypothalamus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

cGMP‐Producing, Atrial Natriuretic Factor‐Responding Cells in the Rat Brain

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Publisher
Wiley
Copyright
Copyright © 1989 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.1989.tb00351.x
Publisher site
See Article on Publisher Site

Abstract

Using an in vitro incubation method, we stimulated cGMP production in rat brain slices by rat ANF‐(103–126). The localization of the cells responding to this ANF stimulation with an increase in cGMP production was studied by cGMP immunocytochemistry. ANF‐responding cells were found in specific loci throughout the central nervous system of the rat. Regions containing the highest number of these cells were: the olfactory bulb, the lateral septum, the bed nucleus of the accessory olfactory tract, the mediobasal amygdala, the central grey area, the medial vestibular nucleus, and the nucleus of the solitary tract. Scattered ANF‐responding, cGMP‐immunoreactive cells were found in the hippocampus, the cingulate cortex, the ventral pallidum, the medial preoptic area, and the endopeduncular nucleus. ANF‐responding cells in these areas had the same morphology, that is, multipolar with numerous processes. The nature of these ANF‐responding cells was studied by sequential staining with an antiserum against glial fibrillary acidic protein (GFAP). In the hippocampus it was demonstrated that all ANF‐responding cells are astroglial cells. However, not all astroglial cells in this area showed a cGMP response, demonstrating a regional heterogeneity. ANF‐responding cells, having the appearance of neuronal cell bodies, could be found in the subfornical organ, and the hypothalamic paraventricular nucleus. Fibres producing cGMP immunoreactivity in response to ANF were found in the median preoptic nucleus, the medial preoptic area, and the dorsal hypothalamus.

Journal

European Journal of NeuroscienceWiley

Published: Sep 1, 1989

References

  • Atrial natriuretic peptide regulates release of Na+‐K+‐ATPase inhibitor from rat brain
    Crabos, Crabos; Ausiello, Ausiello; Haupert, Haupert; Cantiello, Cantiello
  • Atrial natriuretic factors stimulate accumulation and efflux of cyclic GMP in C6–2B rat glioma and PC12 rat pheochromocytoma cell cultures
    Fiscus, Fiscus; Robles, Robles; Waldman, Waldman; Murad, Murad
  • Atrial natriuretic hormones raise the level of cyclic GMP in neural cell lines
    Friedl, Friedl; Harmening, Harmening; Hamprecht, Hamprecht
  • Evidence for lack of a role of cGMP in effect of alpha‐hANP on aldosterone inhibition
    Matsuoka, Matsuoka; Ishii, Ishii; Hirata, Hirata; Atarashi, Atarashi; Sugimoto, Sugimoto; Kangawa, Kangawa; Matsuo, Matsuo
  • Effects of atrial natriuretic polypeptide on rat hypothalamic neurones in vitro
    Okuya, Okuya; Yamashita, Yamashita

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