Abstract— The incorporation of (3H)leucine into proteins of neuron‐enriched and glia‐enriched fractions was studied after various periods of anoxia produced in vitro in rabbit brain. Protein synthesis was carried out with brain slices, and the cellular fractions were subsequently separated with Ficoll density gradient ultracentrifugation. Leucine incorporation decreased promptly even at the early stage of cerebral anoxia (less than 10 min) and the incorporation in the glial fraction declined at least as much as the incorporation in the neuronal fraction. To substantiate that these changes were based on functional alteration of post‐transcriptional or translational mechanism at the polysomal level, polysomes were isolated from anoxic brain slices and polypeptide synthesis was carried out in vitro. The decrease of polypeptide synthesis correlated well with the decline of leucine incorporation in brain slices. Polypeptide synthesis decreased sharply between the 10‐min and the 20‐min anoxic period and a corresponding decrease of the polysomal peak was observed on the polysomal aggregation profile. This study indicated rapid and diffuse inhibition of protein synthesis in cerebral anoxia both in the neuronal and glial elements, the inhibition being based on the alteration of the mechanism for polypeptide synthesis at the polysomal level.
Journal of Neurochemistry – Wiley
Published: Aug 1, 1976
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