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CD‐ROM review

CD‐ROM review Borowitz M, Silberman M. Cases in flow cytometry. Charlottesville: Carden Jennings Publishing Co., Ltd. In the rapidly expanding field of hematologic neoplasia the immunophenotypic analysis of normal and malignant hemopoietic cells by flow cytometry plays a major role in the diagnosis, classification and monitoring of disease. This analysis, however, is not an automated task. It is a complex procedure that requires the use of many reagents in multicolor combinations, a detailed examination of the resulting graphical data, and a thorough characterization of relevant cell subpopulations. Adequate data analysis is critical in the recognition and characterization of neoplastic elements and demands a profound knowledge of the diverse origin and differentiation stages of the cells residing in the hemopoietic tissue. Numerous traditional publications describe immunophenotypic changes observed in marrow and other tissues containing leukemia, lymphoma and related disorders. Some even provide examples of these changes. However, the existing publications only offer limited help to those interested in learning the specific steps involved in data analysis, data display or recognition and characterization of cells of interest, and professionals have to rely mostly on their own experience to understand the process. The interactive CD‐ROM Cases in flow cytometry by Borowitz and Silberman offers many answers to those who wish to understand how data are analyzed in samples with hemopoietic malignancies. This is a remarkable creation that takes advantage of the electronic digital media to provide a unique environment for a large collection of well‐documented and illustrated cases offered in a tutorial format that is fun to read and easy to follow. Each case is presented with a brief clinical history, the antibodies used, and selected two‐parameter multicolor annotated dot plots that can be magnified with a mouse click. A discussion of the diagnostic elements follows with an explanation of findings and summary of disease diagnosis and antigen profile of the abnormal cells detected. All cases presented were analyzed by 3‐ or 4‐color‐conjugated antibodies using a red blood cell lyse method. Correlated CD45 and side scatter analysis is used in most instances to recognize abnormal populations although in some cases other markers are used for identifying critical cells. Each case includes multiple graphs and explanations for the strategies used in the selection of specific antibody combinations. The data analysis is correctly based on pattern recognition rather than on ill‐defined percent positive results. Each pertinent subpopulation of interest is visually dissected out and assigned a distinct color in the dot plots, an approach that facilitates their identification and allows the observer to “follow” the important cells in the various 2‐parameter displays. Most cases are also graced with photographic images of excellent quality for morphologic correlations. All cases are uniformly well organized and clearly presented. The cases are grouped into 4 sections. The first section presents basic concepts and includes 3‐color analysis of a normal marrow and 8 cases of typical leukemias. The second section is quite innovative. It is designated as “Interesting Cases” and contains 15 cases where the reader is given pertinent clinical data, a set of critical flow cytometric images with a list of files containing the corresponding reagent combination, and a link to morphologic images. The reader is then asked to submit a diagnosis. After entering the answer, the reader receives a response and is further oriented by appropriate links to additional graphs, an informative discussion and a final diagnosis and antigen profile. The third section illustrates examples of samples containing minimal disease. These cases demonstrate the power of flow cytometry in the detection of low level neoplastic involvement among abundant normal cells, and emphasize the importance of knowing in detail the phenotype of normal hemopoietic elements. Finally, the fourth section presents 9 cases studied with four‐color antibody combinations and includes appropriate discussions of the value of using this multiparametric approach to reduce the number of tubes required and to define unique cell populations. All cases include a summary with a diagnosis, the immunophenotype of the abnormal cells and a link to the corresponding list mode data accessible to the reader for off‐line analysis. To complement the above the authors also include a helpful Hematopathology Antigen Library with a list of applicable CDs, the corresponding antibody name, their expression in normal and neoplastic cells, the antigen function and pertinent comments. The innovative approach used in this CD‐ROM represents a new concept in education and training in flow cytometry and hematopathology that is informative and enjoyable. It is a valuable resource that should be of interest to the non‐initiated and the experienced alike. Above all, what makes this CD‐ROM extremely useful and distinguishes it from all other publications on this subject is the inclusion of list mode data linked to each case presented, which the readers can interactively analyze on their own. Although some may not completely agree with the number or combination of antibodies chosen, these data undoubtedly represent a most precious material and an extraordinary resource. In summary, I highly recommend exploring this CD‐ROM, which I endorse as a unique learning experience. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytometry Wiley

CD‐ROM review

Cytometry , Volume 46 (1) – Feb 15, 2001

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Publisher
Wiley
Copyright
Copyright © 2001 Wiley‐Liss, Inc.
ISSN
1552-4922
eISSN
1552-4930
DOI
10.1002/1097-0320(20010215)46:1<67::AID-CYTO1042>3.3.CO;2-C
Publisher site
See Article on Publisher Site

Abstract

Borowitz M, Silberman M. Cases in flow cytometry. Charlottesville: Carden Jennings Publishing Co., Ltd. In the rapidly expanding field of hematologic neoplasia the immunophenotypic analysis of normal and malignant hemopoietic cells by flow cytometry plays a major role in the diagnosis, classification and monitoring of disease. This analysis, however, is not an automated task. It is a complex procedure that requires the use of many reagents in multicolor combinations, a detailed examination of the resulting graphical data, and a thorough characterization of relevant cell subpopulations. Adequate data analysis is critical in the recognition and characterization of neoplastic elements and demands a profound knowledge of the diverse origin and differentiation stages of the cells residing in the hemopoietic tissue. Numerous traditional publications describe immunophenotypic changes observed in marrow and other tissues containing leukemia, lymphoma and related disorders. Some even provide examples of these changes. However, the existing publications only offer limited help to those interested in learning the specific steps involved in data analysis, data display or recognition and characterization of cells of interest, and professionals have to rely mostly on their own experience to understand the process. The interactive CD‐ROM Cases in flow cytometry by Borowitz and Silberman offers many answers to those who wish to understand how data are analyzed in samples with hemopoietic malignancies. This is a remarkable creation that takes advantage of the electronic digital media to provide a unique environment for a large collection of well‐documented and illustrated cases offered in a tutorial format that is fun to read and easy to follow. Each case is presented with a brief clinical history, the antibodies used, and selected two‐parameter multicolor annotated dot plots that can be magnified with a mouse click. A discussion of the diagnostic elements follows with an explanation of findings and summary of disease diagnosis and antigen profile of the abnormal cells detected. All cases presented were analyzed by 3‐ or 4‐color‐conjugated antibodies using a red blood cell lyse method. Correlated CD45 and side scatter analysis is used in most instances to recognize abnormal populations although in some cases other markers are used for identifying critical cells. Each case includes multiple graphs and explanations for the strategies used in the selection of specific antibody combinations. The data analysis is correctly based on pattern recognition rather than on ill‐defined percent positive results. Each pertinent subpopulation of interest is visually dissected out and assigned a distinct color in the dot plots, an approach that facilitates their identification and allows the observer to “follow” the important cells in the various 2‐parameter displays. Most cases are also graced with photographic images of excellent quality for morphologic correlations. All cases are uniformly well organized and clearly presented. The cases are grouped into 4 sections. The first section presents basic concepts and includes 3‐color analysis of a normal marrow and 8 cases of typical leukemias. The second section is quite innovative. It is designated as “Interesting Cases” and contains 15 cases where the reader is given pertinent clinical data, a set of critical flow cytometric images with a list of files containing the corresponding reagent combination, and a link to morphologic images. The reader is then asked to submit a diagnosis. After entering the answer, the reader receives a response and is further oriented by appropriate links to additional graphs, an informative discussion and a final diagnosis and antigen profile. The third section illustrates examples of samples containing minimal disease. These cases demonstrate the power of flow cytometry in the detection of low level neoplastic involvement among abundant normal cells, and emphasize the importance of knowing in detail the phenotype of normal hemopoietic elements. Finally, the fourth section presents 9 cases studied with four‐color antibody combinations and includes appropriate discussions of the value of using this multiparametric approach to reduce the number of tubes required and to define unique cell populations. All cases include a summary with a diagnosis, the immunophenotype of the abnormal cells and a link to the corresponding list mode data accessible to the reader for off‐line analysis. To complement the above the authors also include a helpful Hematopathology Antigen Library with a list of applicable CDs, the corresponding antibody name, their expression in normal and neoplastic cells, the antigen function and pertinent comments. The innovative approach used in this CD‐ROM represents a new concept in education and training in flow cytometry and hematopathology that is informative and enjoyable. It is a valuable resource that should be of interest to the non‐initiated and the experienced alike. Above all, what makes this CD‐ROM extremely useful and distinguishes it from all other publications on this subject is the inclusion of list mode data linked to each case presented, which the readers can interactively analyze on their own. Although some may not completely agree with the number or combination of antibodies chosen, these data undoubtedly represent a most precious material and an extraordinary resource. In summary, I highly recommend exploring this CD‐ROM, which I endorse as a unique learning experience.

Journal

CytometryWiley

Published: Feb 15, 2001

There are no references for this article.