CATION MODULATION OF SYNAPTOSOMAL RESPIRATION

CATION MODULATION OF SYNAPTOSOMAL RESPIRATION Abstract— Synaptosomes were prepared from the cerebral cortex of the adult rat by a rapid technique, involving the use of centrifugation in a Ficoll‐sucrose discontinuous gradient. Adequate respiratory control ratios were obtained with glutamate and succinate plus rotenone. The addition of Na+ to the incubation medium stimulated synaptosomal, State‐4 respiration, with a half‐maximal response at 15 mM Na+. The stimulation by Na+ was inhibited by atractylate, oligomycin, ouabain or EDTA. A cooperative interaction between Na+ and low concentrations of Mg2+ was observed. A significant proportion (39 per cent) of the total Na‐K ATPase (EC 3.6.1.4) activity in the discontinuous gradient was localized in the synaptosomal fraction. In the absence of exogenous Mg2+, Na+ induced a 64 per cent stimulation of the synaptosomal ATPase activity which was sensitive to ouabain. Such stimulation of ATP hydrolysis would account for the formation of increased amounts of ADP, with consequent recycling to ATP through adequately controlled oxidative phosphorylation. These observations demonstrate a significant role for transmembrane cationic gradients in the control of synaptosomal respiration and mitochondrial oxidative phosphorylation. The preparation exhibits moderate respiratory control and should prove useful in studies of integrated mitochondrial oxidative metabolism and neuronal membrane function. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

CATION MODULATION OF SYNAPTOSOMAL RESPIRATION

Journal of Neurochemistry, Volume 19 (5) – May 1, 1972

Loading next page...
 
/lp/wiley/cation-modulation-of-synaptosomal-respiration-XdGo1v2VM0
Publisher
Wiley
Copyright
Copyright © 1972 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1972.tb01456.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— Synaptosomes were prepared from the cerebral cortex of the adult rat by a rapid technique, involving the use of centrifugation in a Ficoll‐sucrose discontinuous gradient. Adequate respiratory control ratios were obtained with glutamate and succinate plus rotenone. The addition of Na+ to the incubation medium stimulated synaptosomal, State‐4 respiration, with a half‐maximal response at 15 mM Na+. The stimulation by Na+ was inhibited by atractylate, oligomycin, ouabain or EDTA. A cooperative interaction between Na+ and low concentrations of Mg2+ was observed. A significant proportion (39 per cent) of the total Na‐K ATPase (EC 3.6.1.4) activity in the discontinuous gradient was localized in the synaptosomal fraction. In the absence of exogenous Mg2+, Na+ induced a 64 per cent stimulation of the synaptosomal ATPase activity which was sensitive to ouabain. Such stimulation of ATP hydrolysis would account for the formation of increased amounts of ADP, with consequent recycling to ATP through adequately controlled oxidative phosphorylation. These observations demonstrate a significant role for transmembrane cationic gradients in the control of synaptosomal respiration and mitochondrial oxidative phosphorylation. The preparation exhibits moderate respiratory control and should prove useful in studies of integrated mitochondrial oxidative metabolism and neuronal membrane function.

Journal

Journal of NeurochemistryWiley

Published: May 1, 1972

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off