Catecholamines in equine and bovine plasmas

Catecholamines in equine and bovine plasmas Pharmacol. Themp. 5,279-284, 1982. Catecholamines in equine and bovine plasmas GREGORY E. HARDEE, J I N WANG LAI, SUSAN D. SEMRAD* & CYNTHIA M. TRIM* Department of Pharmaceutics, School of Pharmacy and *Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, U.S.A. A sensitive method for the determination of norepinephrine, epinephrine and dopamine in equine and bovine plasmas is described. The appearance of endogenous compounds particular to equine and bovine plasmas interfere with the published assay methods and require modification of the chromatographic conditions. An in situ ion-pair formation with the compound 3,4-hydroxybenzylamine requires the use of isoproterenol as an internal standard. The extraction procedure which involves the adsorption of the catecholamines by alumina was modified to give 70%-80% recoveries for most compounds. The electrochemical detection of these compounds approaches a detection limit of 40 pg/ml for all but dopamine in bovine plasma which was much higher (250 pg/ml) due to poor extraction. Catecholamine values for various selected bull, cow and horse samples are presented. Additionally several endogenous compounds separated by this method are identified but not quantified. described the simultaneous determination of the catecholamines and many important metabolites in rat serum, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Veterinary Pharmacology & Therapeutics Wiley

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Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0140-7783
eISSN
1365-2885
DOI
10.1111/j.1365-2885.1982.tb00443.x
Publisher site
See Article on Publisher Site

Abstract

Pharmacol. Themp. 5,279-284, 1982. Catecholamines in equine and bovine plasmas GREGORY E. HARDEE, J I N WANG LAI, SUSAN D. SEMRAD* & CYNTHIA M. TRIM* Department of Pharmaceutics, School of Pharmacy and *Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, U.S.A. A sensitive method for the determination of norepinephrine, epinephrine and dopamine in equine and bovine plasmas is described. The appearance of endogenous compounds particular to equine and bovine plasmas interfere with the published assay methods and require modification of the chromatographic conditions. An in situ ion-pair formation with the compound 3,4-hydroxybenzylamine requires the use of isoproterenol as an internal standard. The extraction procedure which involves the adsorption of the catecholamines by alumina was modified to give 70%-80% recoveries for most compounds. The electrochemical detection of these compounds approaches a detection limit of 40 pg/ml for all but dopamine in bovine plasma which was much higher (250 pg/ml) due to poor extraction. Catecholamine values for various selected bull, cow and horse samples are presented. Additionally several endogenous compounds separated by this method are identified but not quantified. described the simultaneous determination of the catecholamines and many important metabolites in rat serum,

Journal

Journal of Veterinary Pharmacology & TherapeuticsWiley

Published: Dec 1, 1982

References

  • Radioenzymic measurement of norepinephrine, epinephrine, and dopamine: stability enzymic activity and sensitivity
    Saar, Saar; Bachmann, Bachmann; Gordon, Gordon

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