Pharmacol. Themp. 5,279-284, 1982. Catecholamines in equine and bovine plasmas GREGORY E. HARDEE, J I N WANG LAI, SUSAN D. SEMRAD* & CYNTHIA M. TRIM* Department of Pharmaceutics, School of Pharmacy and *Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, U.S.A. A sensitive method for the determination of norepinephrine, epinephrine and dopamine in equine and bovine plasmas is described. The appearance of endogenous compounds particular to equine and bovine plasmas interfere with the published assay methods and require modification of the chromatographic conditions. An in situ ion-pair formation with the compound 3,4-hydroxybenzylamine requires the use of isoproterenol as an internal standard. The extraction procedure which involves the adsorption of the catecholamines by alumina was modified to give 70%-80% recoveries for most compounds. The electrochemical detection of these compounds approaches a detection limit of 40 pg/ml for all but dopamine in bovine plasma which was much higher (250 pg/ml) due to poor extraction. Catecholamine values for various selected bull, cow and horse samples are presented. Additionally several endogenous compounds separated by this method are identified but not quantified. described the simultaneous determination of the catecholamines and many important metabolites in rat serum,
Journal of Veterinary Pharmacology & Therapeutics – Wiley
Published: Dec 1, 1982
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera