Candida albicans survival and biofilm formation under starvation conditions

Candida albicans survival and biofilm formation under starvation conditions Aim To investigate the survival and biofilm formation capacity of Candida albicans in starvation and under anaerobic conditions. Methodology Candida albicans growth and survival were monitored in vitro for up to 8 months. Fungal suspensions from late exponential, stationary and starvation phases were incubated on human dentine, polystyrene and glass slides. Scanning electron microscopy (SEM) was used to observe the process of biofilm formation. 2,3‐bis(2‐Methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxyanilide inner salt (XTT) reduction assay was performed to quantify the biofilm formation capability, and confocal laser scanning microscopy (CLSM) was used to study and make semi‐quantitative comparisons of the ultrastructure of biofilms formed on human dentine. ‘XTT bioactivity’ and ‘COMSTAT results’ were analysed by two‐way analysis of variance (ANOVA) and one‐way ANOVA, respectively. Results Candida albicans survived for over six months. SEM demonstrated that starving C. albicans produced mature biofilms on different substrata. C. albicans of the same growth phase incubated on human dentine displayed significantly higher biofilm formation capability than on polystyrene or glass slides (P < 0.05). Biofilm formation capability by starving cells was significantly lower than that in exponential or stationary phases (P < 0.05). CLSM revealed that biofilms formed by starvation‐phase cells were less complex, had a higher roughness coefficient and surface/volume ratio (P < 0.05). Conclusions Candida albicans cells can survive and form biofilms in anaerobic and nutrient‐limited conditions and may pose a treatment challenge. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Endodontic Journal Wiley

Candida albicans survival and biofilm formation under starvation conditions

Loading next page...
 
/lp/wiley/candida-albicans-survival-and-biofilm-formation-under-starvation-vnn0N4KGf6
Publisher
Wiley
Copyright
Copyright © 2013 International Endodontic Journal
ISSN
0143-2885
eISSN
1365-2591
DOI
10.1111/j.1365-2591.2012.02094.x
Publisher site
See Article on Publisher Site

Abstract

Aim To investigate the survival and biofilm formation capacity of Candida albicans in starvation and under anaerobic conditions. Methodology Candida albicans growth and survival were monitored in vitro for up to 8 months. Fungal suspensions from late exponential, stationary and starvation phases were incubated on human dentine, polystyrene and glass slides. Scanning electron microscopy (SEM) was used to observe the process of biofilm formation. 2,3‐bis(2‐Methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxyanilide inner salt (XTT) reduction assay was performed to quantify the biofilm formation capability, and confocal laser scanning microscopy (CLSM) was used to study and make semi‐quantitative comparisons of the ultrastructure of biofilms formed on human dentine. ‘XTT bioactivity’ and ‘COMSTAT results’ were analysed by two‐way analysis of variance (ANOVA) and one‐way ANOVA, respectively. Results Candida albicans survived for over six months. SEM demonstrated that starving C. albicans produced mature biofilms on different substrata. C. albicans of the same growth phase incubated on human dentine displayed significantly higher biofilm formation capability than on polystyrene or glass slides (P < 0.05). Biofilm formation capability by starving cells was significantly lower than that in exponential or stationary phases (P < 0.05). CLSM revealed that biofilms formed by starvation‐phase cells were less complex, had a higher roughness coefficient and surface/volume ratio (P < 0.05). Conclusions Candida albicans cells can survive and form biofilms in anaerobic and nutrient‐limited conditions and may pose a treatment challenge.

Journal

International Endodontic JournalWiley

Published: Jan 1, 2013

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off