Calcium‐mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture

Calcium‐mobilizing and electrophysiological effects of bradykinin on cortical astrocyte... The possible consequences of activating bradykinin (Bk) receptors on identified astrocyte subtypes derived from rat cortex have been investigated in terms of: 1) mobilization of intracellular Ca2+; and (2) electrophysiological response. Bk induced a rapid, transient rise in intracellular Ca2+ in 63% of cortical type‐1‐like astrocytes and 44% of type‐2 astrocytes tested. Experiments involving Ca2+‐free conditions suggested that the release occurred largely from internal stores in both astrocyte subtypes. Bk receptor activation resulted in an inward current in approximately 10% of each astrocyte subtype tested using the whole‐cell patch clamp technique. The use of perforated patch recording confirmed a similar population of cells responsive to Bk. The Bk‐induced current was associated with a decrease in membrane conductance. The reversal potential was close to the K+equilibrium potential, consistent with the closure of K+ channels. The current demonstrated similar EC50values and Hill coefficients and also a marked degree of desensitization for both astrocyte subtypes. Recordings from type‐1‐like astrocytes indicated that the receptor mediating the electrophysiological response was of the B2 subtype. The data were consistent with the functional translation of Bk receptor occupation to physiological responses in distinct sub‐populations of cortical type‐1‐like and type‐2 astrocytes. © 1993 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glia Wiley

Calcium‐mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture

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Publisher
Wiley
Copyright
Copyright © 1993 Wiley‐Liss, Inc.
ISSN
0894-1491
eISSN
1098-1136
DOI
10.1002/glia.440090405
pmid
8112820
Publisher site
See Article on Publisher Site

Abstract

The possible consequences of activating bradykinin (Bk) receptors on identified astrocyte subtypes derived from rat cortex have been investigated in terms of: 1) mobilization of intracellular Ca2+; and (2) electrophysiological response. Bk induced a rapid, transient rise in intracellular Ca2+ in 63% of cortical type‐1‐like astrocytes and 44% of type‐2 astrocytes tested. Experiments involving Ca2+‐free conditions suggested that the release occurred largely from internal stores in both astrocyte subtypes. Bk receptor activation resulted in an inward current in approximately 10% of each astrocyte subtype tested using the whole‐cell patch clamp technique. The use of perforated patch recording confirmed a similar population of cells responsive to Bk. The Bk‐induced current was associated with a decrease in membrane conductance. The reversal potential was close to the K+equilibrium potential, consistent with the closure of K+ channels. The current demonstrated similar EC50values and Hill coefficients and also a marked degree of desensitization for both astrocyte subtypes. Recordings from type‐1‐like astrocytes indicated that the receptor mediating the electrophysiological response was of the B2 subtype. The data were consistent with the functional translation of Bk receptor occupation to physiological responses in distinct sub‐populations of cortical type‐1‐like and type‐2 astrocytes. © 1993 Wiley‐Liss, Inc.

Journal

GliaWiley

Published: Dec 1, 1993

References

  • Inositol 1,4,5‐trisphosphate and diacylglycerol mimic bradykinin effects on mouse neuroblastoma X rat glioma hybrid cells
    Brown, Brown; Higashida, Higashida
  • Identification of bradykinin B 2 binding sites on cultured cortical astrocytes
    Cholewinski, Cholewinski; Stephens, Stephens; MacDermott, MacDermott; Wilkin, Wilkin
  • Cerebral type 2 astroglia are heterogenous with respect to their ability to respond to neuroligands linked to calcium mobilization
    Dave, Dave; Gordon, Gordon; McCarthy, McCarthy
  • Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts
    Estacion, Estacion
  • Identification of neuropeptide Y receptors in cultured astrocytes from neonatal rat brain
    Gimpl, Gimpl; Kirchhoff, Kirchhoff; Leng, Leng; Kettenmann, Kettenmann
  • Muscarinic activation of ionic current measured by a new whole‐cell recording method
    Horn, Horn; Marty, Marty
  • Perforated patch recording
    Korn, Korn; Marty, Marty; Connor, Connor; Horn, Horn
  • Expression of functional bradykinin receptors in Xenopus oocytes
    Phillips, Phillips; Conder, Conder; Bevan, Bevan; McIntyre, McIntyre; Webb, Webb
  • Activation of substance P receptors leads to membrane potential responses in cultured astrocytes
    Wienrich, Wienrich; Kettenmann, Kettenmann

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