Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence

Building a mutant resource for the study of disease resistance in rice reveals the pivotal role... SUMMARY In Arabidopsis, gene expression studies and analysis of knock‐out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up‐regulation of the negative regulator OsWRKY28 and the down‐regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Plant Pathology Wiley

Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence

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Publisher
Wiley
Copyright
© 2011 INRA. MOLECULAR PLANT PATHOLOGY © 2011 BSPP AND BLACKWELL PUBLISHING LTD
ISSN
1464-6722
eISSN
1364-3703
DOI
10.1111/j.1364-3703.2011.00731.x
Publisher site
See Article on Publisher Site

Abstract

SUMMARY In Arabidopsis, gene expression studies and analysis of knock‐out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up‐regulation of the negative regulator OsWRKY28 and the down‐regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences.

Journal

Molecular Plant PathologyWiley

Published: Jan 1, 2012

References

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