Broad‐spectrum protein biosensors for class‐specific detection of antibiotics

Broad‐spectrum protein biosensors for class‐specific detection of antibiotics The dramatically increasing prevalence of multi‐drug‐resistant human pathogenic bacteria and related mortality requires two key actions: (i) decisive initiatives for the detection of novel antibiotics and (ii) a global ban for use of antibiotics as growth promotants in stock farming. Both key actions entail technology for precise, high‐sensitive detection of antibiotic substances either to detect and validate novel anti‐infective structures or to enforce the non‐use of clinically relevant antibiotics. We have engineered prokaryotic antibiotic response regulators into a molecular biosensor configuration able to detect tetracycline, streptogramin, and macrolide antibiotics in spiked liquids including milk and serum at ng/mL concentrations and up to 2 orders of magnitude below current Swiss and EC threshold values. This broad‐spectrum, class‐specific, biosensor‐based assay has been optimized for use in a storable ready‐to‐use and high‐throughput‐compatible ELISA‐type format. At the center of the assay is an antibiotic sensor protein whose interaction with specific DNA fragments is responsive to a particular class of antibiotics. Binding of biosensor protein to the cognate DNA chemically linked to a solid surface is converted into an immuno‐based colorimetric readout correlating with specific antibiotics concentrations. © 2004 Wiley Periodicals, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Broad‐spectrum protein biosensors for class‐specific detection of antibiotics

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Publisher
Wiley
Copyright
Copyright © 2005 Wiley Periodicals, Inc., A Wiley Company
ISSN
0006-3592
eISSN
1097-0290
D.O.I.
10.1002/bit.20224
Publisher site
See Article on Publisher Site

Abstract

The dramatically increasing prevalence of multi‐drug‐resistant human pathogenic bacteria and related mortality requires two key actions: (i) decisive initiatives for the detection of novel antibiotics and (ii) a global ban for use of antibiotics as growth promotants in stock farming. Both key actions entail technology for precise, high‐sensitive detection of antibiotic substances either to detect and validate novel anti‐infective structures or to enforce the non‐use of clinically relevant antibiotics. We have engineered prokaryotic antibiotic response regulators into a molecular biosensor configuration able to detect tetracycline, streptogramin, and macrolide antibiotics in spiked liquids including milk and serum at ng/mL concentrations and up to 2 orders of magnitude below current Swiss and EC threshold values. This broad‐spectrum, class‐specific, biosensor‐based assay has been optimized for use in a storable ready‐to‐use and high‐throughput‐compatible ELISA‐type format. At the center of the assay is an antibiotic sensor protein whose interaction with specific DNA fragments is responsive to a particular class of antibiotics. Binding of biosensor protein to the cognate DNA chemically linked to a solid surface is converted into an immuno‐based colorimetric readout correlating with specific antibiotics concentrations. © 2004 Wiley Periodicals, Inc.

Journal

Biotechnology and BioengineeringWiley

Published: Jan 5, 2005

Keywords: broad‐spectrum biosensors; antibiotic detection; multi‐drug‐resistant human pathogenic bacteria

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