Binding of Bodian's Silver and Monoclonal Antibodies to Defined Regions of Human Neurofilament Subunits: Bodian's Silver Reacts with a Highly Charged Unique Domain of Neurofilaments

Binding of Bodian's Silver and Monoclonal Antibodies to Defined Regions of Human Neurofilament... Abstract: Cleavage at cysteine and chymotrypsin digestion were applied to two human neurofilament (NF) sub‐units, low‐ and high‐molecular‐weight NF (NF‐L and NF‐H), to locate the regions reacting with Bodian's silver stain and with several monoclonal antibodies, including NF‐specific antibodies and one that recognizes all intermediate filaments (anti‐IFA). Our findings indicate that whereas anti‐IFA recognizes the highly conserved rod domain, all the NF‐specific antibodies, as well as Bodian's silver, react with the carboxy‐terminal tailpiece of NF subunits. The silver binding sites in NF‐L are located in a carboxy‐terminal 12‐Kd chymotrypsin fragment, a highly charged, unique domain of NF. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Binding of Bodian's Silver and Monoclonal Antibodies to Defined Regions of Human Neurofilament Subunits: Bodian's Silver Reacts with a Highly Charged Unique Domain of Neurofilaments

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1986.tb12977.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Cleavage at cysteine and chymotrypsin digestion were applied to two human neurofilament (NF) sub‐units, low‐ and high‐molecular‐weight NF (NF‐L and NF‐H), to locate the regions reacting with Bodian's silver stain and with several monoclonal antibodies, including NF‐specific antibodies and one that recognizes all intermediate filaments (anti‐IFA). Our findings indicate that whereas anti‐IFA recognizes the highly conserved rod domain, all the NF‐specific antibodies, as well as Bodian's silver, react with the carboxy‐terminal tailpiece of NF subunits. The silver binding sites in NF‐L are located in a carboxy‐terminal 12‐Kd chymotrypsin fragment, a highly charged, unique domain of NF.

Journal

Journal of NeurochemistryWiley

Published: Feb 1, 1986

References

  • Immunochemical characterization of antisera to rat neurofilament subunits
    Autilio‐Gambetti, Autilio‐Gambetti; Velasco, Velasco; Sipple, Sipple; Gambetti, Gambetti
  • Bulk preparation of CNS cytoskeleton and separation of individual neurofilament proteins by gel filtration: dye‐binding characteristics and amino acid compositions
    Chiu, Chiu; Norton, Norton

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