Basic and clinical science posters: Insulinotropic agents and enteroinsular hormones

Basic and clinical science posters: Insulinotropic agents and enteroinsular hormones P101 [S4K] CPF‐AM1 Mechanisms underlying beneficial actions on pancreatic beta cells in vitro and high‐fat diet‐induced obesity diabetes in vivoYHA ABDEL‐WAHAB, V Musale, BO Owalabi, RC Moffett, OO Ojo, JM Conlon, PR FlattSchool of Biomedical Sciences, Ulster University, Coleraine, UKAims: Previous studies demonstrated insulinotropic activity of CPF‐AM1 (GLGSVLGKALKIGANLL.NH2). In the present study, we extended our investigation on antidiabetic effects of the peptide using the designer novel cationic analogue, [S4K] CPF‐AM1.Methods: Insulin release was assessed using rat (BRIN‐BD11) and human (1.1 B4) pancreatic beta cells. Beta cell proliferation (Ki67) and apoptosis (Tunnel) were examined in BRIN‐BD11 cells. High‐fat mice with obesity diabetes were used to evaluate longer‐term effects of [S4K] CPF‐AM1 (75nmol/kg body weight, i.p.) on blood glucose control and expression of genes involved in insulin signalling and secretion.Results: [S4K] CPF‐AM1 (3μM) produced 321 ± 28% and 260.6 ± 8.07% increase in insulin release from BRIN‐BD11 and 1.1 B4 cells, respectively, compared with control. Culture for 18h with cytokine cocktail [tumour necrosis factor‐α (200U/ml), Interferon gamma (IFNγ) (20U/ml), interleukin‐1β (100U/ml)] decreased proliferation of BRIN‐BD11 cells by 31% compared with control (p < 0.001), whereas [S4K] CPF‐AM1 (10−6M) treated cells exhibited a 33% increase in proliferation. Tunnel‐positive cells were increased by 272% by 18‐h cytokine cocktail treatment but http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetic Medicine Wiley

Basic and clinical science posters: Insulinotropic agents and enteroinsular hormones

Loading next page...
 
/lp/wiley/basic-and-clinical-science-posters-insulinotropic-agents-and-DSrfZweag8
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Diabetic Medicine © 2018 Diabetes UK
ISSN
0742-3071
eISSN
1464-5491
D.O.I.
10.1111/dme.15_13571
Publisher site
See Article on Publisher Site

Abstract

P101 [S4K] CPF‐AM1 Mechanisms underlying beneficial actions on pancreatic beta cells in vitro and high‐fat diet‐induced obesity diabetes in vivoYHA ABDEL‐WAHAB, V Musale, BO Owalabi, RC Moffett, OO Ojo, JM Conlon, PR FlattSchool of Biomedical Sciences, Ulster University, Coleraine, UKAims: Previous studies demonstrated insulinotropic activity of CPF‐AM1 (GLGSVLGKALKIGANLL.NH2). In the present study, we extended our investigation on antidiabetic effects of the peptide using the designer novel cationic analogue, [S4K] CPF‐AM1.Methods: Insulin release was assessed using rat (BRIN‐BD11) and human (1.1 B4) pancreatic beta cells. Beta cell proliferation (Ki67) and apoptosis (Tunnel) were examined in BRIN‐BD11 cells. High‐fat mice with obesity diabetes were used to evaluate longer‐term effects of [S4K] CPF‐AM1 (75nmol/kg body weight, i.p.) on blood glucose control and expression of genes involved in insulin signalling and secretion.Results: [S4K] CPF‐AM1 (3μM) produced 321 ± 28% and 260.6 ± 8.07% increase in insulin release from BRIN‐BD11 and 1.1 B4 cells, respectively, compared with control. Culture for 18h with cytokine cocktail [tumour necrosis factor‐α (200U/ml), Interferon gamma (IFNγ) (20U/ml), interleukin‐1β (100U/ml)] decreased proliferation of BRIN‐BD11 cells by 31% compared with control (p < 0.001), whereas [S4K] CPF‐AM1 (10−6M) treated cells exhibited a 33% increase in proliferation. Tunnel‐positive cells were increased by 272% by 18‐h cytokine cocktail treatment but

Journal

Diabetic MedicineWiley

Published: Jan 1, 2018

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial