Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity

Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody... IntroductionChinese hamster ovary (CHO) cells are the most widely used mammalian host cell lines for the commercial production of recombinant therapeutic proteins, including monoclonal antibodies (mAbs). Over the past two decades, the growing demand for therapeutic mAbs has provided a challenge for the process of developing mass production of high quality mAbs.Endoplasmic reticulum (ER), the central part of the secretory pathways in eukaryotic cells, is responsible for controlling the quality of secreted and resident proteins through the regulation of protein translocation, protein folding, and early post‐translational modifications. A number of physiological conditions such as oxidative stress, hypoglycemia, acidosis, and thermal instability can disturb the ER functions, which triggers ER stress. Prolonged ER stress induces apoptotic cell death.Oxidative stress refers to an imbalance between the production of reactive oxygen species (ROS) – such as hydrogen peroxide (H2O2), superoxide anion, and hydroxyl radicals – and their destruction by the antioxidant defense system. ROS accumulate endogenously as by‐products of oxidative phosphorylation and energy metabolism within mitochondria and protein folding and disulfide bond formation in the ER during cell cultures. Antioxidants reduce the oxidative stress level and suppress the apoptotic cell death by scavenging oxygen free radicals, inhibiting chain reaction of oxidation, and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1860-6768
eISSN
1860-7314
D.O.I.
10.1002/biot.201700425
Publisher site
See Article on Publisher Site

Abstract

IntroductionChinese hamster ovary (CHO) cells are the most widely used mammalian host cell lines for the commercial production of recombinant therapeutic proteins, including monoclonal antibodies (mAbs). Over the past two decades, the growing demand for therapeutic mAbs has provided a challenge for the process of developing mass production of high quality mAbs.Endoplasmic reticulum (ER), the central part of the secretory pathways in eukaryotic cells, is responsible for controlling the quality of secreted and resident proteins through the regulation of protein translocation, protein folding, and early post‐translational modifications. A number of physiological conditions such as oxidative stress, hypoglycemia, acidosis, and thermal instability can disturb the ER functions, which triggers ER stress. Prolonged ER stress induces apoptotic cell death.Oxidative stress refers to an imbalance between the production of reactive oxygen species (ROS) – such as hydrogen peroxide (H2O2), superoxide anion, and hydroxyl radicals – and their destruction by the antioxidant defense system. ROS accumulate endogenously as by‐products of oxidative phosphorylation and energy metabolism within mitochondria and protein folding and disulfide bond formation in the ER during cell cultures. Antioxidants reduce the oxidative stress level and suppress the apoptotic cell death by scavenging oxygen free radicals, inhibiting chain reaction of oxidation, and

Journal

Biotechnology JournalWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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