Baicalein disturbs the morphological plasticity and motility of breast adenocarcinoma cells depending on the tumor microenvironment

Baicalein disturbs the morphological plasticity and motility of breast adenocarcinoma cells... During tumor invasion, cancer cells change their morphology and mode of migration based on communication with the surrounding environment. Numerous studies have indicated that paracrine interactions from non‐neoplastic cells impact the migratory and invasive properties of cancer cells. Thus, these interactions are potential targets for anticancer therapies. In this study, we showed that the flavones member baicalein suppresses the motility of breast cancer cells that is promoted by paracrine interactions. First, we identified laminin‐332 (LN‐332) as a principle paracrine factor in conditioned medium from mammary epithelium‐derived MCF10A cells that regulates the morphology and motility of breast adenocarcinoma MDA‐MB‐231 cells. Then, we carried out a morphology‐based screen for small compounds, which showed that baicalein suppressed the morphological changes and migratory activity of MDA‐MB‐231 cells that were induced by conditioned medium from MCF10A cells and LN‐332. We also found that baicalein caused narrower and incomplete lamellipodia formation in conditioned medium‐treated MDA‐MB‐231 cells, although actin dynamics downstream of Rho family small GTPases were unaffected. These results suggest the importance of mammary epithelial cells in the cancer microenvironment promoting the migratory activity of breast adenocarcinoma cells and show a novel mechanism through which baicalein inhibits cancer cell motility. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes to Cells Wiley

Baicalein disturbs the morphological plasticity and motility of breast adenocarcinoma cells depending on the tumor microenvironment

Loading next page...
 
/lp/wiley/baicalein-disturbs-the-morphological-plasticity-and-motility-of-breast-3xhdrHQ3mj
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd
ISSN
1356-9597
eISSN
1365-2443
D.O.I.
10.1111/gtc.12584
Publisher site
See Article on Publisher Site

Abstract

During tumor invasion, cancer cells change their morphology and mode of migration based on communication with the surrounding environment. Numerous studies have indicated that paracrine interactions from non‐neoplastic cells impact the migratory and invasive properties of cancer cells. Thus, these interactions are potential targets for anticancer therapies. In this study, we showed that the flavones member baicalein suppresses the motility of breast cancer cells that is promoted by paracrine interactions. First, we identified laminin‐332 (LN‐332) as a principle paracrine factor in conditioned medium from mammary epithelium‐derived MCF10A cells that regulates the morphology and motility of breast adenocarcinoma MDA‐MB‐231 cells. Then, we carried out a morphology‐based screen for small compounds, which showed that baicalein suppressed the morphological changes and migratory activity of MDA‐MB‐231 cells that were induced by conditioned medium from MCF10A cells and LN‐332. We also found that baicalein caused narrower and incomplete lamellipodia formation in conditioned medium‐treated MDA‐MB‐231 cells, although actin dynamics downstream of Rho family small GTPases were unaffected. These results suggest the importance of mammary epithelial cells in the cancer microenvironment promoting the migratory activity of breast adenocarcinoma cells and show a novel mechanism through which baicalein inhibits cancer cell motility.

Journal

Genes to CellsWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off