ABSTRACT: In a 3‐month period four episodes of bacterial sepsis related to contaminated random donor platelet concentrates were observed. Evaluation incriminated extended (5 or more days) platelet storage. To determine if platelets stored for longer periods were likely to have greater bacterial contamination, platelet concentrates were innoculated with bacteria and daily quantitative bacteriology was performed. In vitro studies using polyolefin bags indicated contamination could occur with a single organism of Staphylococcus epidermidis. By 72 hours, platelet concentrates contained from 103 to 108 organisms per 0.1 ml depending on the inoculum size. By 6 days all inoculated units contained 107 to 109 organisms per 0.1 ml.
Transfusion – Wiley
Published: Jul 8, 1986
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