Astrocytes and Bergmann glia as an important site of nitric oxide synthase I

Astrocytes and Bergmann glia as an important site of nitric oxide synthase I In the central nervous system nitric oxide appears to be critically involved in a number of physiological and pathological processes. Although there is convincing evidence for expression of nitric oxide synthase in cultured glial cells, demonstration of this enzyme in glial cells in situ remained largely unsatisfactory. In the present study we applied immunostaining to freeze‐dried sections of snap‐frozen hippocampi and cerebella of rats and to sections of freeze‐dried brain tissue in order to minimize diffusion artefacts and thus to obtain more precise information about the true in situ localization of nitric oxide synthase. Here we show that astrocytes and Bergmann glia react strongly with antibodies raised against cerebellar nitric oxide synthase and against a type I nitric oxide synthase‐specific C‐terminal peptide, respectively. This finding was further substantiated by histochemical localization of NADPH‐diaphorase activity in astrocytes and Bergmann glia as well as by immunoreactivity of both types of glia cells with antibodies to the NADPH‐delivering enzyme glucose‐6‐phosphate dehydrogenase. We conclude, that astrocytes are important sites of nitric oxide synthase I in brain, suggesting that these cells might use nitric oxide as gaseous messenger molecule for various aspects of glia‐neuron signalling. © 1996 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glia Wiley

Astrocytes and Bergmann glia as an important site of nitric oxide synthase I

Glia, Volume 16 (2) – Feb 1, 1996

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Publisher
Wiley
Copyright
Copyright © 1996 Wiley‐Liss, Inc.
ISSN
0894-1491
eISSN
1098-1136
DOI
10.1002/(SICI)1098-1136(199602)16:2<165::AID-GLIA8>3.0.CO;2-2
Publisher site
See Article on Publisher Site

Abstract

In the central nervous system nitric oxide appears to be critically involved in a number of physiological and pathological processes. Although there is convincing evidence for expression of nitric oxide synthase in cultured glial cells, demonstration of this enzyme in glial cells in situ remained largely unsatisfactory. In the present study we applied immunostaining to freeze‐dried sections of snap‐frozen hippocampi and cerebella of rats and to sections of freeze‐dried brain tissue in order to minimize diffusion artefacts and thus to obtain more precise information about the true in situ localization of nitric oxide synthase. Here we show that astrocytes and Bergmann glia react strongly with antibodies raised against cerebellar nitric oxide synthase and against a type I nitric oxide synthase‐specific C‐terminal peptide, respectively. This finding was further substantiated by histochemical localization of NADPH‐diaphorase activity in astrocytes and Bergmann glia as well as by immunoreactivity of both types of glia cells with antibodies to the NADPH‐delivering enzyme glucose‐6‐phosphate dehydrogenase. We conclude, that astrocytes are important sites of nitric oxide synthase I in brain, suggesting that these cells might use nitric oxide as gaseous messenger molecule for various aspects of glia‐neuron signalling. © 1996 Wiley‐Liss, Inc.

Journal

GliaWiley

Published: Feb 1, 1996

References

  • Homocysteate, an excitatory transmitter candidate localized in glia
    Grandes, Grandes; Do, Do; Morino, Morino; Cuénod, Cuénod; Streit, Streit
  • Nitric oxide and synaptic function
    Schuman, Schuman; Madison, Madison

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