Association of cytoplasmic free Ca 2+ gradients with subcellular organelles

Association of cytoplasmic free Ca 2+ gradients with subcellular organelles 10.1002/jcp.1041500321.abs Previous investigations have identified gradients of intracellular free (Ca2+)i (Ca2+)i in the cytoplasm of human fibroblasts. In this study we have compared the spatial distribution of these gradients with the subcellular distribution of cytoplasmic organelles. Using the Ca2+‐sensitive dye fura‐2 and organelle‐specific fluorescent dyes, we have found that the highest Ca2+ concentrations are found in the perinuclear cytoplasm and that these regions co‐localize with the Golgi apparatus. The area occupied by the endoplasmic reticulum, which includes the Golgi region plus an adjacent area, is also significantly elevated above the average cellular (Ca2+)i. Most mitochondria are located in regions different from those with the highest (Ca2+)i. A variety of phenomena which could have given rise to artifactual (Ca2+)i gradients have been ruled out, including compartmentalization of fura‐2 in subcellular organelles, incomplete hydrolysis of fura‐2AM esters, and the presence of pH gradients which might change the Ca2+ binding characteristics of fura‐2. The existence of gradients in (Ca2+)i between ER and Golgi containing regions of the cytoplasm supports the hypothesis (Sambrook:between Cell 61:197–199, 1990) that the traffic of membrane bound vesicles from ER to Golgi is directed by local variations in (Ca2+)i. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Association of cytoplasmic free Ca 2+ gradients with subcellular organelles

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Publisher
Wiley
Copyright
Copyright © 1992 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.1041500321
pmid
1537888
Publisher site
See Article on Publisher Site

Abstract

10.1002/jcp.1041500321.abs Previous investigations have identified gradients of intracellular free (Ca2+)i (Ca2+)i in the cytoplasm of human fibroblasts. In this study we have compared the spatial distribution of these gradients with the subcellular distribution of cytoplasmic organelles. Using the Ca2+‐sensitive dye fura‐2 and organelle‐specific fluorescent dyes, we have found that the highest Ca2+ concentrations are found in the perinuclear cytoplasm and that these regions co‐localize with the Golgi apparatus. The area occupied by the endoplasmic reticulum, which includes the Golgi region plus an adjacent area, is also significantly elevated above the average cellular (Ca2+)i. Most mitochondria are located in regions different from those with the highest (Ca2+)i. A variety of phenomena which could have given rise to artifactual (Ca2+)i gradients have been ruled out, including compartmentalization of fura‐2 in subcellular organelles, incomplete hydrolysis of fura‐2AM esters, and the presence of pH gradients which might change the Ca2+ binding characteristics of fura‐2. The existence of gradients in (Ca2+)i between ER and Golgi containing regions of the cytoplasm supports the hypothesis (Sambrook:between Cell 61:197–199, 1990) that the traffic of membrane bound vesicles from ER to Golgi is directed by local variations in (Ca2+)i.

Journal

Journal of Cellular PhysiologyWiley

Published: Mar 1, 1992

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