Asparaginase gene expression is regulated in a complex spatial and temporal pattern in nitrogen‐sink tissues

Asparaginase gene expression is regulated in a complex spatial and temporal pattern in... Asparagine is an important nitrogen transport compound in higher plants. Mobilized asparagine is deamidated by the enzyme asparaginase to aspartate and ammonia, which are major sources of organic nitrogen for plant growth and development. Little is known about the role of asparagine catabolism during plant development as the enzyme is difficult to assay, although it is known that asparaginase activity is located in tissues undergoing rapid growth and development, particularly in young leaves and developing seeds. In tobacco plants containing an asparaginase‐GUS gene fusion, GUS expression was specifically located in young developing tissues such as the apical meristem and expanding leaves. In mature plants, GUS activity was found predominantly in various tissues of the developing seed. In lupin, where up to 80% of the fixed nitrogen is exported as asparagine to the developing seed, GUS activity was also located in developing tissues such as the apical meristem, and in specific tissue types in the developing seed previously shown to contain asparaginase activity. All of the tissues in which asparaginase expression occurred have previously been shown to contain localized high levels of asparagine. This study therefore demonstrates an important role for transcriptional control of an asparaginase gene in regulating asparaginase levels in N‐sink tissues. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Asparaginase gene expression is regulated in a complex spatial and temporal pattern in nitrogen‐sink tissues

The Plant Journal, Volume 5 (5) – May 1, 1994

Loading next page...
 
/lp/wiley/asparaginase-gene-expression-is-regulated-in-a-complex-spatial-and-0oxQ4mAt20
Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1111/j.1365-313X.1994.00695.x
Publisher site
See Article on Publisher Site

Abstract

Asparagine is an important nitrogen transport compound in higher plants. Mobilized asparagine is deamidated by the enzyme asparaginase to aspartate and ammonia, which are major sources of organic nitrogen for plant growth and development. Little is known about the role of asparagine catabolism during plant development as the enzyme is difficult to assay, although it is known that asparaginase activity is located in tissues undergoing rapid growth and development, particularly in young leaves and developing seeds. In tobacco plants containing an asparaginase‐GUS gene fusion, GUS expression was specifically located in young developing tissues such as the apical meristem and expanding leaves. In mature plants, GUS activity was found predominantly in various tissues of the developing seed. In lupin, where up to 80% of the fixed nitrogen is exported as asparagine to the developing seed, GUS activity was also located in developing tissues such as the apical meristem, and in specific tissue types in the developing seed previously shown to contain asparaginase activity. All of the tissues in which asparaginase expression occurred have previously been shown to contain localized high levels of asparagine. This study therefore demonstrates an important role for transcriptional control of an asparaginase gene in regulating asparaginase levels in N‐sink tissues.

Journal

The Plant JournalWiley

Published: May 1, 1994

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off