Ascorbic, Dehydroascorbic and Isoascorbic Acid Simultaneous Determinations by Reverse Phase Ion Interaction HPLC

Ascorbic, Dehydroascorbic and Isoascorbic Acid Simultaneous Determinations by Reverse Phase Ion... ABSTRACT Detection was by UV absorbance (after pre‐column derivatization of dehydroascorbic acid with 1,2‐phenylenediamine). When absent, isoascorbic acid could be used as internal standard. Isocratic separation was accomplished in 11 min using the eluent, methanol‐water (5:95, v/v) containing potassium dihydrogen phosphate (50 mM) and the counterion hexadecyltrimethylammonium bromide (5 mM). Sample preparation steps using Sep‐pak C18 cartridge were minimal. Ten ppm could be detected for each compound with good reproducibility (c.v. <2%). The method was used to determine vitamin C content in selected foods and beverages. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Food Science Wiley

Ascorbic, Dehydroascorbic and Isoascorbic Acid Simultaneous Determinations by Reverse Phase Ion Interaction HPLC

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Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1147
eISSN
1750-3841
D.O.I.
10.1111/j.1365-2621.1992.tb05527.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT Detection was by UV absorbance (after pre‐column derivatization of dehydroascorbic acid with 1,2‐phenylenediamine). When absent, isoascorbic acid could be used as internal standard. Isocratic separation was accomplished in 11 min using the eluent, methanol‐water (5:95, v/v) containing potassium dihydrogen phosphate (50 mM) and the counterion hexadecyltrimethylammonium bromide (5 mM). Sample preparation steps using Sep‐pak C18 cartridge were minimal. Ten ppm could be detected for each compound with good reproducibility (c.v. <2%). The method was used to determine vitamin C content in selected foods and beverages.

Journal

Journal of Food ScienceWiley

Published: Mar 1, 1992

References

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