Application of PCR‐SSCP to Species Identification of Fishery Products

Application of PCR‐SSCP to Species Identification of Fishery Products A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand conformation polymorphism (SSCP) to get species‐specific patterns of single‐stranded DNA (ssDNA). DNA strands were separated by polyacrylamide gel electrophoresis and visualised by silver staining. Differentiation between four eel species was possible. Each of three types of sturgeon caviar gave a characteristic pattern of ssDNA. Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of the Science of Food and Agriculture Wiley

Application of PCR‐SSCP to Species Identification of Fishery Products

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Publisher
Wiley
Copyright
Copyright © 1997 SCI
ISSN
0022-5142
eISSN
1097-0010
D.O.I.
10.1002/(SICI)1097-0010(199705)74:1<35::AID-JSFA765>3.0.CO;2-2
Publisher site
See Article on Publisher Site

Abstract

A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand conformation polymorphism (SSCP) to get species‐specific patterns of single‐stranded DNA (ssDNA). DNA strands were separated by polyacrylamide gel electrophoresis and visualised by silver staining. Differentiation between four eel species was possible. Each of three types of sturgeon caviar gave a characteristic pattern of ssDNA. Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI.

Journal

Journal of the Science of Food and AgricultureWiley

Published: May 1, 1997

References

  • Screening techniques for detecting allelic variation in DNA sequences
    Lessa, Lessa; Applebaum, Applebaum
  • Caviare: proximate composition, amino acid content and identification of fish species
    Rehbein, Rehbein
  • Electrophoretic techniques for species identification of fishery products
    Rehbein, Rehbein

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