Application of an electrophoretic methodology for the identification of low molecular weight proteins in foods

Application of an electrophoretic methodology for the identification of low molecular weight... Identification and characterisation of food proteins are core features of food allergy research. Current methods used to identify allergenic proteins in food have insufficient resolution and are unable to delect low molecular weight proteins. In this study we report the use of a simple SDS‐PAGE method which allows resolution of small proteins. We have subsequently applied this method and reported presence of low molecular weight proteins in a range of hydrolysed milk formulae (Nutramigen, Pregistimil, Alfare, Pepti‐Junior and Pregomin), and crude peanut protein extract. The molecular weight distribution for the peanut extract and the hydrolysates ranged between 5‐200kDa and 2‐17kDa respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pediatric Allergy and Immunology Wiley

Application of an electrophoretic methodology for the identification of low molecular weight proteins in foods

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Publisher
Wiley
Copyright
Copyright © 1996 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0905-6157
eISSN
1399-3038
DOI
10.1111/j.1399-3038.1996.tb00128.x
Publisher site
See Article on Publisher Site

Abstract

Identification and characterisation of food proteins are core features of food allergy research. Current methods used to identify allergenic proteins in food have insufficient resolution and are unable to delect low molecular weight proteins. In this study we report the use of a simple SDS‐PAGE method which allows resolution of small proteins. We have subsequently applied this method and reported presence of low molecular weight proteins in a range of hydrolysed milk formulae (Nutramigen, Pregistimil, Alfare, Pepti‐Junior and Pregomin), and crude peanut protein extract. The molecular weight distribution for the peanut extract and the hydrolysates ranged between 5‐200kDa and 2‐17kDa respectively.

Journal

Pediatric Allergy and ImmunologyWiley

Published: Nov 1, 1996

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