Antibody responses to GalC in severe and complicated childhood Guillain‐Barré syndrome

Antibody responses to GalC in severe and complicated childhood Guillain‐Barré syndrome Dear Editor,We recently presented a case series of seven children who developed severe and complicated Guillain‐Barré syndrome (GBS) after infection with Mycoplasma pneumoniae (Mp) (Meyer Sauteur et al., ). The disease was rapidly progressive and severe: one died, four had clinically defined central nervous system (CNS) involvement, and five required mechanical ventilation. Given the often relatively benign disease course of GBS after this specific type of infection (Meyer Sauteur et al., ) the severe and complicated GBS in this series was rather unexpected. As we recently demonstrated that pediatric and adult GBS after Mp infection is associated with antibodies against galactocerebroside (GalC) (Meyer Sauteur et al., ) we investigated if anti‐GalC antibodies were also present in these children with severe GBS.Sera were tested for immunoglobulin (Ig)M and IgG antibodies to GM1, GM2, GD1a, GD1b, GQ1b, and GalC (all from Sigma–Aldrich, Zwijndrecht, The Netherlands) as previously described (Ang et al., ; Kuijf et al., ; Meyer Sauteur et al., ). Cerebrospinal fluid (CSF) was tested for IgM and IgG to GalC at 1:10 dilution. Optical densities (ODs) at 490 nm from uncoated wells (containing ethanol) were subtracted from glycolipid‐coated wells. Cut‐off values (0.05 for IgG and 0.03 for IgM) were http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of the Peripheral Nervous System Wiley

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 Peripheral Nerve Society
ISSN
1085-9489
eISSN
1529-8027
D.O.I.
10.1111/jns.12243
Publisher site
See Article on Publisher Site

Abstract

Dear Editor,We recently presented a case series of seven children who developed severe and complicated Guillain‐Barré syndrome (GBS) after infection with Mycoplasma pneumoniae (Mp) (Meyer Sauteur et al., ). The disease was rapidly progressive and severe: one died, four had clinically defined central nervous system (CNS) involvement, and five required mechanical ventilation. Given the often relatively benign disease course of GBS after this specific type of infection (Meyer Sauteur et al., ) the severe and complicated GBS in this series was rather unexpected. As we recently demonstrated that pediatric and adult GBS after Mp infection is associated with antibodies against galactocerebroside (GalC) (Meyer Sauteur et al., ) we investigated if anti‐GalC antibodies were also present in these children with severe GBS.Sera were tested for immunoglobulin (Ig)M and IgG antibodies to GM1, GM2, GD1a, GD1b, GQ1b, and GalC (all from Sigma–Aldrich, Zwijndrecht, The Netherlands) as previously described (Ang et al., ; Kuijf et al., ; Meyer Sauteur et al., ). Cerebrospinal fluid (CSF) was tested for IgM and IgG to GalC at 1:10 dilution. Optical densities (ODs) at 490 nm from uncoated wells (containing ethanol) were subtracted from glycolipid‐coated wells. Cut‐off values (0.05 for IgG and 0.03 for IgM) were

Journal

Journal of the Peripheral Nervous SystemWiley

Published: Jan 1, 2018

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