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Anti‐ROS‐DNA monoclonal antibody as molecular probe for oxidative DNA damage

Anti‐ROS‐DNA monoclonal antibody as molecular probe for oxidative DNA damage Modification of 400 bp (approximate size) calf thymus DNA with OH radical resulted in lowered Tm, modification of thymine (58.3%), guanine (20%) and single strand breaks. Monoclonal antibodies (mAb) generated against ROS‐DNA were of IgG1 subclass. The mAb showed strong binding to ROS‐DNA and ROS‐modified bases and polymers, in particular, of thymine. The mAb, therefore, preferentially recognizes ROS‐modified epitopes on nucleic acids. Distinct binding to DNA isolated from aged, but not from normal humans by the monoclonal antibdy was observed. The antibody effectively recognized oxidative lesions in DNA from cancer patients. These studies demonstrate the potential application of the mAb as an immunochemical probe to detect oxidative DNA lesions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png IUBMB Life Wiley

Anti‐ROS‐DNA monoclonal antibody as molecular probe for oxidative DNA damage

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 1997 International Union of Biochemistry and Molecular Biology
ISSN
1521-6543
eISSN
1521-6551
DOI
10.1080/15216549700205051
Publisher site
See Article on Publisher Site

Abstract

Modification of 400 bp (approximate size) calf thymus DNA with OH radical resulted in lowered Tm, modification of thymine (58.3%), guanine (20%) and single strand breaks. Monoclonal antibodies (mAb) generated against ROS‐DNA were of IgG1 subclass. The mAb showed strong binding to ROS‐DNA and ROS‐modified bases and polymers, in particular, of thymine. The mAb, therefore, preferentially recognizes ROS‐modified epitopes on nucleic acids. Distinct binding to DNA isolated from aged, but not from normal humans by the monoclonal antibdy was observed. The antibody effectively recognized oxidative lesions in DNA from cancer patients. These studies demonstrate the potential application of the mAb as an immunochemical probe to detect oxidative DNA lesions.

Journal

IUBMB LifeWiley

Published: Dec 1, 1997

Keywords: Reactive oxygen species; oxidative DNA damage; monoclonal antibody; antibody probe; cancer; aging

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