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Anomalies in sedimentation. II. Testing the entanglement hypothesis

Anomalies in sedimentation. II. Testing the entanglement hypothesis Zones of T2 DNA were sedimented through uniform solutions of T7 DNA to determine if the smaller DNA molecules would become entangled in the larger. No entanglement could be demonstrated even at high DNA concentrations. It is suggested that molecular entanglement is not responsible for the sudden loss of DNA from solution which occurs in high centrifugal fields. This communication also includes observations on the effects of rotor speed on the sedimentation behavior of DNA in high centrifugal fields, distortion of zone shape at high concentrations, and hydrodynamic interactions between DNA and MS2 bacteriophage particles. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Anomalies in sedimentation. II. Testing the entanglement hypothesis

Biopolymers , Volume 12 (4) – Apr 1, 1973

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References (14)

Publisher
Wiley
Copyright
Copyright © 1973 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.1973.360120415
pmid
4695681
Publisher site
See Article on Publisher Site

Abstract

Zones of T2 DNA were sedimented through uniform solutions of T7 DNA to determine if the smaller DNA molecules would become entangled in the larger. No entanglement could be demonstrated even at high DNA concentrations. It is suggested that molecular entanglement is not responsible for the sudden loss of DNA from solution which occurs in high centrifugal fields. This communication also includes observations on the effects of rotor speed on the sedimentation behavior of DNA in high centrifugal fields, distortion of zone shape at high concentrations, and hydrodynamic interactions between DNA and MS2 bacteriophage particles.

Journal

BiopolymersWiley

Published: Apr 1, 1973

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