Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme‐linked immunosorbent assay and gas chromatography/tandem mass spectrometry

Analysis of estrogenic hormones in municipal wastewater effluent and surface water using... Ahough the estrogenichormones177β‐estradiol and 17α‐ethinyl estradiol can be quantified in polluted waters by gas chromatography/mass spectrometry (GC/MS) and gas chromatography/tandem mass spectrometry (GC/MS/MS), the compounds often are present at concentrations below detection limits. Enzyme‐linked immunosorbent assays (ELISAs) provide a sensitive and robust means of quantifying estrogenic hormones in wastewater effluents and surface waters. Results from ELISA analysis of estrogenic hormones in secondary wastewater effluent indicate concentrations comparable to those that cause vitellogenesis in fish. Confirmatory analyses by GC/MS/MS are consistent with ELISA results. Effluent filtration, using sand filtration or microfiltration, removes approx. 70% of the hormones from secondary effluent, while advanced treatment, using reverse osmosis, removes more than 95% of hormones. The detection limits for estrogenic hormones are approx. 0.1 ng/L in wastewater effluent and 0.05 ng/L in surface water. The ELISA technique provides a relatively simple and practical method of assessing the fate of estrogenic hormones in engineered and natural systems. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Toxicology & Chemistry Wiley

Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme‐linked immunosorbent assay and gas chromatography/tandem mass spectrometry

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Publisher
Wiley
Copyright
Copyright © 2001 SETAC
ISSN
0730-7268
eISSN
1552-8618
D.O.I.
10.1002/etc.5620200114
Publisher site
See Article on Publisher Site

Abstract

Ahough the estrogenichormones177β‐estradiol and 17α‐ethinyl estradiol can be quantified in polluted waters by gas chromatography/mass spectrometry (GC/MS) and gas chromatography/tandem mass spectrometry (GC/MS/MS), the compounds often are present at concentrations below detection limits. Enzyme‐linked immunosorbent assays (ELISAs) provide a sensitive and robust means of quantifying estrogenic hormones in wastewater effluents and surface waters. Results from ELISA analysis of estrogenic hormones in secondary wastewater effluent indicate concentrations comparable to those that cause vitellogenesis in fish. Confirmatory analyses by GC/MS/MS are consistent with ELISA results. Effluent filtration, using sand filtration or microfiltration, removes approx. 70% of the hormones from secondary effluent, while advanced treatment, using reverse osmosis, removes more than 95% of hormones. The detection limits for estrogenic hormones are approx. 0.1 ng/L in wastewater effluent and 0.05 ng/L in surface water. The ELISA technique provides a relatively simple and practical method of assessing the fate of estrogenic hormones in engineered and natural systems.

Journal

Environmental Toxicology & ChemistryWiley

Published: Jan 1, 2001

References

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