Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Analysis of cytosolic ionized calcium variation in polymorphonuclear leukocytes using flow cytometry and Indo‐1 AM

Analysis of cytosolic ionized calcium variation in polymorphonuclear leukocytes using flow... The present report describes the increase in cytosolic‐free calcium levels (Ca2+i) induced by chemoattractants in polymorphonuclear leukocytes (PMN) using the new fluorescent Ca2+ chelator Indo‐1 AM. Increases in cytosolic Ca2+ were measured by flow cytometry. With this approach, 98% of PMN were found to respond to F‐Met‐Leu‐Phe (FMLP) and leukotriene B4 (LTB4) stimulation. Although both substances induced a rapid and relatively homogeneous rise in Ca2+i, only FMLP gave a sustained Ca2+i rise, whereas that induced by LTB4 appeared transient. In addition, the combined use of wide‐angle light scatter and electronic cell volume measurement allowed analysis of Ca2+i variations in the total peripheral blood cell population. The supernatant of a human fibrous histiocytoma cell line (GCT) was able to increase Ca2+ release in PMN. This activity may be ascribed to a new granulocytic activation factor, as neither human recombinant interleukin‐1α (IL‐1α), granulocyte‐colony stimulating factor (G‐CSF), nor granulocyte‐macrophage‐colony stimulating factor (GM‐CSF), which were present in this supernatant, were able to induce a Ca2+i rise in PMN. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytometry Part A Wiley

Analysis of cytosolic ionized calcium variation in polymorphonuclear leukocytes using flow cytometry and Indo‐1 AM

Cytometry Part A , Volume 10 (2) – Mar 1, 1989

Loading next page...
 
/lp/wiley/analysis-of-cytosolic-ionized-calcium-variation-in-polymorphonuclear-mP7He2XBCF

References (36)

Publisher
Wiley
Copyright
Copyright © 1989 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1552-4922
eISSN
1552-4930
DOI
10.1002/cyto.990100207
pmid
2540938
Publisher site
See Article on Publisher Site

Abstract

The present report describes the increase in cytosolic‐free calcium levels (Ca2+i) induced by chemoattractants in polymorphonuclear leukocytes (PMN) using the new fluorescent Ca2+ chelator Indo‐1 AM. Increases in cytosolic Ca2+ were measured by flow cytometry. With this approach, 98% of PMN were found to respond to F‐Met‐Leu‐Phe (FMLP) and leukotriene B4 (LTB4) stimulation. Although both substances induced a rapid and relatively homogeneous rise in Ca2+i, only FMLP gave a sustained Ca2+i rise, whereas that induced by LTB4 appeared transient. In addition, the combined use of wide‐angle light scatter and electronic cell volume measurement allowed analysis of Ca2+i variations in the total peripheral blood cell population. The supernatant of a human fibrous histiocytoma cell line (GCT) was able to increase Ca2+ release in PMN. This activity may be ascribed to a new granulocytic activation factor, as neither human recombinant interleukin‐1α (IL‐1α), granulocyte‐colony stimulating factor (G‐CSF), nor granulocyte‐macrophage‐colony stimulating factor (GM‐CSF), which were present in this supernatant, were able to induce a Ca2+i rise in PMN.

Journal

Cytometry Part AWiley

Published: Mar 1, 1989

Keywords: ; ; ; ; ;

There are no references for this article.