An optimized method for isolating and sequencing large (CA/GT)n (n > 40) microsatellites from genomic DNA

An optimized method for isolating and sequencing large (CA/GT)n (n > 40) microsatellites from... C. RICO, I. RICO and G. HEWITT Schoot of Bmlogicul Sciencps, Uniuersity of East Anglia, Nomich, Norfolk NR4 7Tf, UK Microsatellites are defined as short tandem repeats with repeat motives of one to five nucleotides. They are often hypervariable in repeat number and length and thus, provide a useful source of polymorphic genetic markers for the construction of linkage maps, paternity testing, and population genetic studies (Weber & May 1989; Tautz 1989; Estoup et al. 1993, and references therein). The one drawback of the technique is that a set of polymorphic microsatellite loci has tp be isolated and sequenced for each species and computer searches of published sequences are possible in only in few vertebrate species (viz. human and mouse) (Stallings et al. 1991).The cloning, isolation and characterization of microsatellites is well documented (Rassman et al. 1991; Pandolfo 1992; Santibanez rt al. 1993). However, the determination of the sequences flanking microsatellites is most laborious particularly when larger than 40 repeats, perhaps due to the formation of secondary structures (Stallings et al. 1991).Most microsatellite polymorphism analyses are based upon (CAI,?/ (GT),,dinucleotide repeats (Estoup et nl. 1993; Beckmann and Weber 1992), with an average number of repeats of 23 2 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Ecology Wiley

An optimized method for isolating and sequencing large (CA/GT)n (n > 40) microsatellites from genomic DNA

Molecular Ecology, Volume 3 (2) – Apr 1, 1994

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Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0962-1083
eISSN
1365-294X
DOI
10.1111/j.1365-294X.1994.tb00120.x
Publisher site
See Article on Publisher Site

Abstract

C. RICO, I. RICO and G. HEWITT Schoot of Bmlogicul Sciencps, Uniuersity of East Anglia, Nomich, Norfolk NR4 7Tf, UK Microsatellites are defined as short tandem repeats with repeat motives of one to five nucleotides. They are often hypervariable in repeat number and length and thus, provide a useful source of polymorphic genetic markers for the construction of linkage maps, paternity testing, and population genetic studies (Weber & May 1989; Tautz 1989; Estoup et al. 1993, and references therein). The one drawback of the technique is that a set of polymorphic microsatellite loci has tp be isolated and sequenced for each species and computer searches of published sequences are possible in only in few vertebrate species (viz. human and mouse) (Stallings et al. 1991).The cloning, isolation and characterization of microsatellites is well documented (Rassman et al. 1991; Pandolfo 1992; Santibanez rt al. 1993). However, the determination of the sequences flanking microsatellites is most laborious particularly when larger than 40 repeats, perhaps due to the formation of secondary structures (Stallings et al. 1991).Most microsatellite polymorphism analyses are based upon (CAI,?/ (GT),,dinucleotide repeats (Estoup et nl. 1993; Beckmann and Weber 1992), with an average number of repeats of 23 2

Journal

Molecular EcologyWiley

Published: Apr 1, 1994

Keywords: ;

References

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