An electron diffraction study of paramylon storage granules from Euglena gracilis

An electron diffraction study of paramylon storage granules from Euglena gracilis Paramylon storage granules from Euglena gracilis were characterized by electron diffraction techniques using electrons of various accelerating voltages: 2 MV for the thick granules and 100 kV for the thinner ones. Intact granules gave well resolved, characteristic (1→3)‐β‐d glucan fibre diffraction diagrams with the glucan molecular orientation parallel to the longer axis of the granule. Hydrated electron diffraction patterns with better resolution were obtained from thinner granules by examination at low temperatures of quench‐frozen specimens. In this case, the pattern indexed on an hexagonal system with a = b= 1·55 + 0·01 nm and c (fibre axis) = 1·86 ± 0·01 nm. Sections of embedded granules provided single crystal‐like electron diffraction patterns corresponding to various sections of the reciprocal lattice of (1→3)‐β‐d glucan (including the hko section). Finally, by scanning electron microscopy, it was shown that the granules swell on contact with water and take up a characteristic ribbed, pumpkin‐like shape. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Microscopy Wiley

An electron diffraction study of paramylon storage granules from Euglena gracilis

Journal of Microscopy, Volume 121 (2) – Feb 1, 1981

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Publisher
Wiley
Copyright
Copyright © 1981 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-2720
eISSN
1365-2818
DOI
10.1111/j.1365-2818.1981.tb01206.x
Publisher site
See Article on Publisher Site

Abstract

Paramylon storage granules from Euglena gracilis were characterized by electron diffraction techniques using electrons of various accelerating voltages: 2 MV for the thick granules and 100 kV for the thinner ones. Intact granules gave well resolved, characteristic (1→3)‐β‐d glucan fibre diffraction diagrams with the glucan molecular orientation parallel to the longer axis of the granule. Hydrated electron diffraction patterns with better resolution were obtained from thinner granules by examination at low temperatures of quench‐frozen specimens. In this case, the pattern indexed on an hexagonal system with a = b= 1·55 + 0·01 nm and c (fibre axis) = 1·86 ± 0·01 nm. Sections of embedded granules provided single crystal‐like electron diffraction patterns corresponding to various sections of the reciprocal lattice of (1→3)‐β‐d glucan (including the hko section). Finally, by scanning electron microscopy, it was shown that the granules swell on contact with water and take up a characteristic ribbed, pumpkin‐like shape.

Journal

Journal of MicroscopyWiley

Published: Feb 1, 1981

References

  • The triple helical structure of lentinan, a linear β‐(1 → 3)‐d glucan
    Bluhm, T.L.; Sarko, A.
  • Ultrastructural observation of (1 → 3)‐β‐d‐glucan from fungal cell‐walls
    Jelsma, J.; Kreger, D.R.

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