Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Yu, V. Rubio, Na-Yeoun Lee, Sulan Bai, Sun-Young Lee, Sang-Sook Kim, Lijing Liu, Yi-yue Zhang, M. Irigoyen, James Sullivan, Yu Zhang, Ilha Lee, Q. Xie, N. Paek, X. Deng (2008)
COP1 and ELF3 control circadian function and photoperiodic flowering by regulating GI stability.Molecular cell, 32 5
H. McWatters, R. Bastow, Anthony Hall, A. Millar (2000)
The ELF3 zeitnehmer regulates light signalling to the circadian clockNature, 408
Lay-Hong Ang, Sudip Chattopadhyay, N. Wei, T. Oyama, K. Okada, A. Batschauer, X. Deng (1998)
Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development.Molecular cell, 1 2
Guangping Chen, Haidong Huang, O. Fröhlich, Yuan Yang, J. Klein, S. Price, J. Sands (2008)
MDM2 E3 ubiquitin ligase mediates UT-A1 urea transporter ubiquitination and degradation.American journal of physiology. Renal physiology, 295 5
S. Stone, Luis Williams, Lisa Farmer, R. Vierstra, J. Callis (2006)
KEEP ON GOING, a RING E3 Ligase Essential for Arabidopsis Growth and Development, Is Involved in Abscisic Acid Signaling[W]The Plant Cell Online, 18
J. Nie, Melanie McGill, Matt Dermer, S. Dho, Cheryl Wolting, C. McGlade (2002)
LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin‐dependent degradationThe EMBO Journal, 21
L. Johansen, J. Carrington (2001)
Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system.Plant physiology, 126 3
E. Koscianska, K. Kalantidis, K. Wypijewski, J. Sadowski, M. Tabler (2005)
Analysis of RNA Silencing in Agroinfiltrated Leaves of Nicotiana Benthamiana and Nicotiana TabacumPlant Molecular Biology, 59
Xiuren Zhang, Virginia Garretón, N. Chua (2005)
The AIP2 E3 ligase acts as a novel negative regulator of ABA signaling by promoting ABI3 degradation.Genes & development, 19 13
B. Downes, R. Vierstra (2005)
Post-translational regulation in plants employing a diverse set of polypeptide tags.Biochemical Society transactions, 33 Pt 2
T. Wróblewski, A. Tomczak, R. Michelmore (2005)
Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis.Plant biotechnology journal, 3 2
S. Ueki, Benoit Lacroix, A. Krichevsky, S. Lazarowitz, V. Citovsky (2008)
Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardmentNature Protocols, 4
Nathalie Mokrzycki-Issartel, B. Bouchon, Sibille Farrer, Patricia Berland, H. Laparra, J. Madelmont, M. Theisen (2003)
A transient tobacco expression system coupled to MALDI‐TOF‐MS allows validation of the impact of differential targeting on structure and activity of a recombinant therapeutic glycoprotein produced in plantsFEBS Letters, 552
Y. Saijo, James Sullivan, Haiyang Wang, Jianping Yang, Yunping Shen, V. Rubio, Ligeng Ma, U. Hoecker, X. Deng (2003)
The COP1-SPA1 interaction defines a critical step in phytochrome A-mediated regulation of HY5 activity.Genes & development, 17 21
H. Lee, S. Cho, O. Son, Zhengyi Xu, I. Hwang, W. Kim (2009)
Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in Transgenic Arabidopsis Plants[C][W]The Plant Cell Online, 21
J. Kapila, R. Rycke, M. Montagu, G. Angenon (1997)
An Agrobacterium-mediated transient gene expression system for intact leavesPlant Science, 122
Meilyn Rodríguez, N. Ramírez, M. Ayala, F. Freyre, L. Pérez, A. Triguero, C. Mateo, G. Selman-Housein, J. Gavilondo, M. Pujol (2005)
Transient expression in tobacco leaves of an aglycosylated recombinant antibody against the epidermal growth factor receptor.Biotechnology and bioengineering, 89 2
Shengyun Fang, J. Jensen, R. Ludwig, K. Vousden, A. Weissman (2000)
Mdm2 Is a RING Finger-dependent Ubiquitin Protein Ligase for Itself and p53*The Journal of Biological Chemistry, 275
S. Abel, A. Theologis (1994)
Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression.The Plant journal : for cell and molecular biology, 5 3
M. Osterlund, C. Hardtke, N. Wei, X. Deng (2000)
Targeted destabilization of HY5 during light-regulated development of ArabidopsisNature, 405
Karen Hicks, A. Millar, I. Carré, D. Somers, M. Straume, D. Meeks-Wagner, S. Kay (1996)
Conditional Circadian Dysfunction of the Arabidopsis early-flowering 3 MutantScience, 274
M. Goodin, R. Dietzgen, D. Schichnes, S. Ruzin, A. Jackson (2002)
pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves.The Plant journal : for cell and molecular biology, 31 3
O. Voinnet, S. Rivas, P. Mestre, D. Baulcombe (2003)
An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.The Plant journal : for cell and molecular biology, 33 5
P. Corn (2007)
Role of the ubiquitin proteasome system in renal cell carcinomaBMC Biochemistry, 8
R. Chakrabarty, R. Banerjee, Sang-Min Chung, M. Farman, V. Citovsky, S. Hogenhout, T. Tzfira, M. Goodin (2007)
PSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions.Molecular plant-microbe interactions : MPMI, 20 7
Q. Xie, Hui Guo, G. Dallman, Shengyun Fang, A. Weissman, N. Chua (2002)
SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signalsNature, 419
H. Hellmann, M. Estelle (2002)
Plant Development: Regulation by Protein DegradationScience, 297
K. Dreher, J. Callis (2007)
Ubiquitin, hormones and biotic stress in plants.Annals of botany, 99 5
Jingjing Liu, Yiyue Zhang, Genji Qin, T. Tsuge, N. Sakaguchi, Guo-Jie Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, T. Aoyama, A. Oka, Weicai Yang, M. Umeda, Q. Xie, H. Gu, Li-jia Qu (2008)
Targeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis Gametogenesis[W][OA]The Plant Cell Online, 20
E. Turnbull, M. Rosser, D. Cyr (2007)
The role of the UPS in cystic fibrosisBMC Biochemistry, 8
(2010)
The Plant Journal
Hershko (1998)
The ubiquitin systemAnnu. Rev. Biochem., 67
Ligeng Ma, Ying Gao, Li-jia Qu, Zhang-liang Chen, Jinming Li, Hongyu Zhao, X. Deng (2002)
Genomic Evidence for COP1 as a Repressor of Light-Regulated Gene Expression and Development in Arabidopsis Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.004416.The Plant Cell Online, 14
Priyanka Dikshit, N. Jana (2008)
Role of Ubiquitin Protein Ligases in the Pathogenesis of Polyglutamine DiseasesNeurochemical Research, 33
A. Devoto, P. Muskett, K. Shirasu (2003)
Role of ubiquitination in the regulation of plant defence against pathogens.Current opinion in plant biology, 6 4
S. Stone, J. Callis (2007)
Ubiquitin ligases mediate growth and development by promoting protein death.Current opinion in plant biology, 10 6
Yiyue Zhang, Chengwei Yang, Yin Li, Nuoyan Zheng, Hao Chen, Qingzhen Zhao, Ting Gao, Huishan Guo, Q. Xie (2007)
SDIR1 Is a RING Finger E3 Ligase That Positively Regulates Stress-Responsive Abscisic Acid Signaling in Arabidopsis[W]The Plant Cell Online, 19
A. Samach, Jennifer Klenz, S. Kohalmi, E. Risseeuw, G. Haughn, W. Crosby (1999)
The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein required for normal patterning and growth in the floral meristem.The Plant journal : for cell and molecular biology, 20 4
Efficient technique for detection of ubiquitination 903
X. Deng, M. Matsui, N. Wei, D. Wagner, A. Chu, K. Feldmann, P. Quail (1992)
COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domainCell, 71
Pengda Ma, Jinying Liu, Hongxian He, Meiying Yang, Meina Li, Xiaojuan Zhu, Xingzhi Wang (2009)
A Viral Suppressor P1/HC-Pro Increases the GFP Gene Expression in Agrobacterium-mediated Transient AssayApplied Biochemistry and Biotechnology, 158
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate‐specific interactions and ubiquitination play important roles in this pathway. However, due to technical limitations only a few instances of E3 ligase–substrate binding and protein ubiquitination in plants have been directly evidenced. An efficient in vivo and in vitro ubiquitination assay was developed for analysis of protein ubiquitination reactions by agroinfiltration expression of both substrates and E3 ligases in Nicotiana benthamiana. Using a detailed analysis of the well‐known E3 ligase COP1 and its substrate HY5, we demonstrated that this assay allows for fast and reliable detection of the specific interaction between the substrate and the E3 ligase, as well as the effects of MG132 and substrate ubiquitination and degradation. We were able to differentiate between the original and ubiquitinated forms of the substrate in vivo with antibodies to ubiquitin or to the target protein. We also demonstrated that the substrate and E3 ligase proteins expressed by agroinfiltration can be applied to analyze ubiquitination in in vivo or in vitro reactions. In addition, we optimized the conditions for different types of substrate and E3 ligase expression by supplementation with the gene‐silencing suppressor p19 and by time‐courses of sample collection. Finally, by testing different protein extraction buffers, we found that different types of buffer should be used for different ubiquitination analyses. This method should be adaptable to other protein modification studies.
The Plant Journal – Wiley
Published: Mar 1, 2010
Keywords: ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.