Allergen‐Specific IgE Antibodies against Antigenic Components in Cow Milk and Milk Substitutes

Allergen‐Specific IgE Antibodies against Antigenic Components in Cow Milk and Milk Substitutes Crossed radioimmunoelectrophoresis (CRIE) was used to study the presence of scrum IgE against antigenic components of Cow milk in 21 selected milk‐allergic patients. The amount of each IgE specificity was estimated by a scoring system. The milk‐allergic children had mainly IgE against α‐lactalbumin, β‐lactoglobulin, albumin and immuno‐globulin, the four major proteins of bovin whey as well as IgE against three cascin components. A serum pool from 1000 normal adults had IgE against the same whey protein, but in smaller amounts, and no IgE against the casein components. Eight cow milk‐based formulae, commonly used for infant feeding, and goat milk were studied by the same method. It was found that six of the milk substitutes did not differ significantly from cow milk in antibody binding, but the two hydrolysed casein products. Nutramigen® and Pregestimil®, consisted of such small molecules that the rabbit antisera could not precipitate the Indrolysed proteins in the gels on the CRIE plates. It was therefore not possible to study their IgE binding, if any, by this method. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Allergy Wiley

Allergen‐Specific IgE Antibodies against Antigenic Components in Cow Milk and Milk Substitutes

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0105-4538
eISSN
1398-9995
DOI
10.1111/j.1398-9995.1986.tb00275.x
Publisher site
See Article on Publisher Site

Abstract

Crossed radioimmunoelectrophoresis (CRIE) was used to study the presence of scrum IgE against antigenic components of Cow milk in 21 selected milk‐allergic patients. The amount of each IgE specificity was estimated by a scoring system. The milk‐allergic children had mainly IgE against α‐lactalbumin, β‐lactoglobulin, albumin and immuno‐globulin, the four major proteins of bovin whey as well as IgE against three cascin components. A serum pool from 1000 normal adults had IgE against the same whey protein, but in smaller amounts, and no IgE against the casein components. Eight cow milk‐based formulae, commonly used for infant feeding, and goat milk were studied by the same method. It was found that six of the milk substitutes did not differ significantly from cow milk in antibody binding, but the two hydrolysed casein products. Nutramigen® and Pregestimil®, consisted of such small molecules that the rabbit antisera could not precipitate the Indrolysed proteins in the gels on the CRIE plates. It was therefore not possible to study their IgE binding, if any, by this method.

Journal

AllergyWiley

Published: Jan 1, 1986

References

  • Characterization of bovine whey proteins by crossed immunoelectrophoresis
    Løwenstein, Løwenstein; Bjerrum, Bjerrum; Weeke, Weeke; Weeke, Weeke
  • Purification of human IgE and rabbit anti‐human IgE
    Løwenstein, Løwenstein; Weeke, Weeke

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