Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from Brains of Chick Embryos

Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from... Abstract: Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8‐day‐old and 15‐day‐old chick embryos, respectively, were grown for periods between 3 and 19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts were found to contain predominantly the anaerobic isoenzymatic form of LDH (LDH‐H4), and this pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH‐H4, and there was a progressive appearance of all other isoenzymes over an 8‐day period. These results support the hypothesis of a different energy metabolism in neurons and glia. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from Brains of Chick Embryos

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Publisher
Wiley
Copyright
Copyright © 1981 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1981.tb02379.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8‐day‐old and 15‐day‐old chick embryos, respectively, were grown for periods between 3 and 19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts were found to contain predominantly the anaerobic isoenzymatic form of LDH (LDH‐H4), and this pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH‐H4, and there was a progressive appearance of all other isoenzymes over an 8‐day period. These results support the hypothesis of a different energy metabolism in neurons and glia.

Journal

Journal of NeurochemistryWiley

Published: Jan 1, 1981

References

  • Activity and isoenzyme pattern of lactate dehydrogenase in astroblasts cultured from brains of newborn mice
    Nissen, Nissen; Schousboe, Schousboe
  • Attachment and culture of dissociated cells from rat embryo cerebral hemispheres on polylysine‐coated surface
    Yavin, Yavin; Yavin, Yavin

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