Summary ACGT‐containing ABA response elements (ABREs) have been functionally identified in the promoters of various genes. In addition, single copies of ABRE have been found to require a cis‐acting, coupling element to achieve ABA induction. A coupling element 3 (CE3) sequence, originally identified as such in the barley HVA1 promoter, is found approximately 30 bp downstream of motif A (ACGT‐containing ABRE) in the promoter of the Osem gene. The relationship between these two elements was further defined by linker‐scan analyses of a 55 bp fragment of the Osem promoter, which is sufficient for ABA‐responsiveness and VP1 activation. The analyses revealed that both motif A and CE3 sequence were required not only for ABA‐responsiveness but also for VP1 activation. Since the sequences of motif A and CE3 were found to be similar, motif‐exchange experiments were carried out. The experiments demonstrated that motif A and CE3 were interchangeable by each other with respect to both ABA and VP1 regulation. In addition, both sequences were shown to be recognized by a VP1‐interacting, ABA‐responsive bZIP factor TRAB1. These results indicate that ACGT‐containing ABREs and CE3 are functionally equivalent cis‐acting elements. Furthermore, TRAB1 was shown to bind two other non‐ACGT ABREs. Based on these results, all these ABREs including CE3 are proposed to be categorized into a single class of cis‐acting elements.
The Plant Journal – Wiley
Published: Sep 1, 1999
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera