A ten‐color tube with dried antibody reagents for the screening of hematological malignancies

A ten‐color tube with dried antibody reagents for the screening of hematological malignancies INTRODUCTIONThe workflow in clinical flow cytometry (FCM) laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. In Brazil, most clinical laboratories perform FCM immunophenotyping using four combinations of fluorochrome‐conjugated antibodies. Although this strategy has been proven to be efficient for the diagnosis, classification, prognosis, and monitoring of hematological malignancies, advances in instrumentation have enabled the development of new flow cytometers with the ability to detect up to 8 colors, consequently increasing the accuracy and standardization of clinical FCM data.Despite the benefits of analyzing up to 8 colors, FCM laboratories still experience many long‐standing issues, such as the instability of and lot‐to‐lot variations in tandem dyes; difficulties in multicolor compensation; pipetting errors; short shelf life of products; preparation, documentation, and quality assurance of cocktail reagents; and the need for standardization. Moreover, the assays require time‐consuming processes, such as the validation, titration, and monitoring of fluorochrome‐conjugated antibodies.As a result of a partnership with the Flow Cytometry Laboratory at the Department of Pathology in the Laboratory Medicine Program of the University Health Network, Toronto, ON, Canada, in October 2014, we introduced a single tube containing 10 colors and 14 fluorochrome‐conjugated antibodies into our FCM http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Laboratory Hematology Wiley

A ten‐color tube with dried antibody reagents for the screening of hematological malignancies

Loading next page...
 
/lp/wiley/a-ten-color-tube-with-dried-antibody-reagents-for-the-screening-of-lLwu0kkWgG
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons Ltd
ISSN
1751-5521
eISSN
1751-553X
D.O.I.
10.1111/ijlh.12753
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONThe workflow in clinical flow cytometry (FCM) laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. In Brazil, most clinical laboratories perform FCM immunophenotyping using four combinations of fluorochrome‐conjugated antibodies. Although this strategy has been proven to be efficient for the diagnosis, classification, prognosis, and monitoring of hematological malignancies, advances in instrumentation have enabled the development of new flow cytometers with the ability to detect up to 8 colors, consequently increasing the accuracy and standardization of clinical FCM data.Despite the benefits of analyzing up to 8 colors, FCM laboratories still experience many long‐standing issues, such as the instability of and lot‐to‐lot variations in tandem dyes; difficulties in multicolor compensation; pipetting errors; short shelf life of products; preparation, documentation, and quality assurance of cocktail reagents; and the need for standardization. Moreover, the assays require time‐consuming processes, such as the validation, titration, and monitoring of fluorochrome‐conjugated antibodies.As a result of a partnership with the Flow Cytometry Laboratory at the Department of Pathology in the Laboratory Medicine Program of the University Health Network, Toronto, ON, Canada, in October 2014, we introduced a single tube containing 10 colors and 14 fluorochrome‐conjugated antibodies into our FCM

Journal

International Journal of Laboratory HematologyWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off