INTRODUCTIONThe workflow in clinical flow cytometry (FCM) laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. In Brazil, most clinical laboratories perform FCM immunophenotyping using four combinations of fluorochrome‐conjugated antibodies. Although this strategy has been proven to be efficient for the diagnosis, classification, prognosis, and monitoring of hematological malignancies, advances in instrumentation have enabled the development of new flow cytometers with the ability to detect up to 8 colors, consequently increasing the accuracy and standardization of clinical FCM data.Despite the benefits of analyzing up to 8 colors, FCM laboratories still experience many long‐standing issues, such as the instability of and lot‐to‐lot variations in tandem dyes; difficulties in multicolor compensation; pipetting errors; short shelf life of products; preparation, documentation, and quality assurance of cocktail reagents; and the need for standardization. Moreover, the assays require time‐consuming processes, such as the validation, titration, and monitoring of fluorochrome‐conjugated antibodies.As a result of a partnership with the Flow Cytometry Laboratory at the Department of Pathology in the Laboratory Medicine Program of the University Health Network, Toronto, ON, Canada, in October 2014, we introduced a single tube containing 10 colors and 14 fluorochrome‐conjugated antibodies into our FCM
International Journal of Laboratory Hematology – Wiley
Published: Jan 1, 2018
Keywords: ; ; ;
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