INTRODUCTIONThe workflow in clinical flow cytometry (FCM) laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. In Brazil, most clinical laboratories perform FCM immunophenotyping using four combinations of fluorochrome‐conjugated antibodies. Although this strategy has been proven to be efficient for the diagnosis, classification, prognosis, and monitoring of hematological malignancies, advances in instrumentation have enabled the development of new flow cytometers with the ability to detect up to 8 colors, consequently increasing the accuracy and standardization of clinical FCM data.Despite the benefits of analyzing up to 8 colors, FCM laboratories still experience many long‐standing issues, such as the instability of and lot‐to‐lot variations in tandem dyes; difficulties in multicolor compensation; pipetting errors; short shelf life of products; preparation, documentation, and quality assurance of cocktail reagents; and the need for standardization. Moreover, the assays require time‐consuming processes, such as the validation, titration, and monitoring of fluorochrome‐conjugated antibodies.As a result of a partnership with the Flow Cytometry Laboratory at the Department of Pathology in the Laboratory Medicine Program of the University Health Network, Toronto, ON, Canada, in October 2014, we introduced a single tube containing 10 colors and 14 fluorochrome‐conjugated antibodies into our FCM
International Journal of Laboratory Hematology – Wiley
Published: Jan 1, 2018
Keywords: ; ; ;
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera