A Simplified Method for Freezing Human Blood Platelets in Glycerol‐Glucose Using a Statically Controlled Cooling Rate Device

A Simplified Method for Freezing Human Blood Platelets in Glycerol‐Glucose Using a Statically... An inexpensive, statically controlled cooling rate device consisting of a cassette of aluminum plates and cardboard insulation in a plastic bag was used to freeze platelets in liquid nitrogen with glycerol‐glucose as cryopro‐tectant. Depending on the thermocouple location, the cooling rate in the freezing bag varied between 22.1 and 38.6 C per minute, averaging 33.6 ± 1.1 C per minute at the center. The post‐thaw recovery of platelets frozen with this device and reconstituted in plasma averaged 88.6 ± 11.7 per cent, compared to 86.1 ± 9.9 per cent for nonfrozen, but otherwise identically processed platelets. 14C Serotonin uptake after 0.5‐hour incubation was 95.9 ± 1.9 per cent for fresh platelets in platelet‐rich plasma, 92.7 ± 4.4 per cent for nonfrozen processed platelets, and 81.4 ± 11.8 per cent for frozen platelets, increasing to 85.9 ± 7.7 per cent after one‐hour incubation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

A Simplified Method for Freezing Human Blood Platelets in Glycerol‐Glucose Using a Statically Controlled Cooling Rate Device

Transfusion, Volume 19 (3) – May 6, 1979

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Publisher
Wiley
Copyright
1979 AABB
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1046/j.1537-2995.1979.19379204206.x
Publisher site
See Article on Publisher Site

Abstract

An inexpensive, statically controlled cooling rate device consisting of a cassette of aluminum plates and cardboard insulation in a plastic bag was used to freeze platelets in liquid nitrogen with glycerol‐glucose as cryopro‐tectant. Depending on the thermocouple location, the cooling rate in the freezing bag varied between 22.1 and 38.6 C per minute, averaging 33.6 ± 1.1 C per minute at the center. The post‐thaw recovery of platelets frozen with this device and reconstituted in plasma averaged 88.6 ± 11.7 per cent, compared to 86.1 ± 9.9 per cent for nonfrozen, but otherwise identically processed platelets. 14C Serotonin uptake after 0.5‐hour incubation was 95.9 ± 1.9 per cent for fresh platelets in platelet‐rich plasma, 92.7 ± 4.4 per cent for nonfrozen processed platelets, and 81.4 ± 11.8 per cent for frozen platelets, increasing to 85.9 ± 7.7 per cent after one‐hour incubation.

Journal

TransfusionWiley

Published: May 6, 1979

References

  • Morphology and enumeration of human blood platelets
    Brecher, Brecher; Cronkite, Cronkite
  • Statically controlled cooling rate device
    Pert, Pert; Dayian, Dayian

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