A simple rosette assay for demonstration of complement receptor sites using complement‐coated zymosan beads

A simple rosette assay for demonstration of complement receptor sites using complement‐coated... In the present article we describe a simple rosette assay for detection of C3 receptor‐bearing B lymphocytes with complement (C)‐coated zymosan (Zy) beads. Zy coated with murine or human C bound to a distinct population of human and mouse lymphocytes as well as to the majority of lymphoblastoid cells of several human established cell lines. Rosette formation was also observed with human red blood cells, with human monocytes and neutrophils. Experiments with anti‐immunoglobulin sera, with other B and T cell markers and with mouse thymocytes proved that the capacity to bind C‐coated Zy is primarily a feature of B lymphocytes. The following findings suggested that C‐coated Zy is bound via receptor sites for the activated components of C3: (a) Zy coated with C3‐deficient serum failed to bind, (b) comparable percentages of various lymphoid and nonlymphoid cells formed rosettes with C‐coated Zy as well as with antibody and C‐coated sheep red bloodcells, and (c) antibodies against human or murine C3 inhibited the binding of C‐coated Zy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Immunology Wiley

A simple rosette assay for demonstration of complement receptor sites using complement‐coated zymosan beads

European Journal of Immunology, Volume 5 (6) – Jun 1, 1975

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Publisher
Wiley
Copyright
Copyright © 1975 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
0014-2980
eISSN
1521-4141
DOI
10.1002/eji.1830050616
Publisher site
See Article on Publisher Site

Abstract

In the present article we describe a simple rosette assay for detection of C3 receptor‐bearing B lymphocytes with complement (C)‐coated zymosan (Zy) beads. Zy coated with murine or human C bound to a distinct population of human and mouse lymphocytes as well as to the majority of lymphoblastoid cells of several human established cell lines. Rosette formation was also observed with human red blood cells, with human monocytes and neutrophils. Experiments with anti‐immunoglobulin sera, with other B and T cell markers and with mouse thymocytes proved that the capacity to bind C‐coated Zy is primarily a feature of B lymphocytes. The following findings suggested that C‐coated Zy is bound via receptor sites for the activated components of C3: (a) Zy coated with C3‐deficient serum failed to bind, (b) comparable percentages of various lymphoid and nonlymphoid cells formed rosettes with C‐coated Zy as well as with antibody and C‐coated sheep red bloodcells, and (c) antibodies against human or murine C3 inhibited the binding of C‐coated Zy.

Journal

European Journal of ImmunologyWiley

Published: Jun 1, 1975

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