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Nachman Nachman (1968)
Platelet proteinsSeminars in Haematology, 5
R. Dixon, W. Rosse, L. Ebbert (1975)
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(1975)
A look at HLA antisera in the I F T test
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Immunofluorescence Microphotometry for the Detection of Platelet AntibodiesScandinavian Journal of Immunology, 4
Van Cj, Engelfriet Cp (1973)
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Platelet antigens and iso-immunization. Thesis
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A. Rivière, P. Verhoef‐Karssen, A. Bosma, A. Borne, P. Engelfriet (1976)
Specific Antisera Against Human Blood Cells Applicable in the Indirect Immunofluorescence TechniqueScandinavian Journal of Immunology, 5
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H. Denk, M. Fischer, H. Pilgerstorfer, H. Schnack (1969)
Immunfluoreszenzmikrophotometrie zur quantitativen Erfassung antithrombozytärer AutoantikörperThrombosis and Haemostasis, 21
H. Denk, M. Fischer, H. Pilgerstorfer, H. Schnack (1969)
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Mueller‐Eckhardt Mueller‐Eckhardt, Sachs Sachs (1966)
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Denk Denk, Fisher Fisher, Pilgerstorfer Pilgerstorfer, Schnack Schnack (1969)
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C. Boxtel, C. Engelfriet (1973)
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(1969)
Methods used in platelet and leukocyte immunology
Immunofluorescence tests on platelets have always been hampered by nonspecific fluorescence caused by non‐immunological binding of plasma proteins to the platelet membrane. It was found that this could be easily overcome by fixation of the cells with paraformaldehyde (PFA). By using PFA‐fixed platelets, a simple method for the detection of platelet antibodies, the platelet suspension immunofluorescence test (PSIFT) was developed. PFA fixation did not alter or inactivate the platelet antigens tested. Platelet‐reactive antibodies detected specifically with the PSIFT included platelet‐specific agglutinins of the IgM class, non‐agglutinating platelet‐specific antibodies of the IgG class, drug‐dependent platelet antibodies, HLA antibodies, as well as anti‐A and anti‐B antibodies. The sensitivity of the new test was satisfactory, as was its reproducibility. Measurement of platelet immunofluorescence was possible in a continuous flow microfluorometer, making in principle, quantitation of platelet antibodies and antigens possible.
British Journal of Haematology – Wiley
Published: Jun 1, 1978
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