A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies Immunofluorescence tests on platelets have always been hampered by nonspecific fluorescence caused by non‐immunological binding of plasma proteins to the platelet membrane. It was found that this could be easily overcome by fixation of the cells with paraformaldehyde (PFA). By using PFA‐fixed platelets, a simple method for the detection of platelet antibodies, the platelet suspension immunofluorescence test (PSIFT) was developed. PFA fixation did not alter or inactivate the platelet antigens tested. Platelet‐reactive antibodies detected specifically with the PSIFT included platelet‐specific agglutinins of the IgM class, non‐agglutinating platelet‐specific antibodies of the IgG class, drug‐dependent platelet antibodies, HLA antibodies, as well as anti‐A and anti‐B antibodies. The sensitivity of the new test was satisfactory, as was its reproducibility. Measurement of platelet immunofluorescence was possible in a continuous flow microfluorometer, making in principle, quantitation of platelet antibodies and antigens possible. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

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Abstract

Immunofluorescence tests on platelets have always been hampered by nonspecific fluorescence caused by non‐immunological binding of plasma proteins to the platelet membrane. It was found that this could be easily overcome by fixation of the cells with paraformaldehyde (PFA). By using PFA‐fixed platelets, a simple method for the detection of platelet antibodies, the platelet suspension immunofluorescence test (PSIFT) was developed. PFA fixation did not alter or inactivate the platelet antigens tested. Platelet‐reactive antibodies detected specifically with the PSIFT included platelet‐specific agglutinins of the IgM class, non‐agglutinating platelet‐specific antibodies of the IgG class, drug‐dependent platelet antibodies, HLA antibodies, as well as anti‐A and anti‐B antibodies. The sensitivity of the new test was satisfactory, as was its reproducibility. Measurement of platelet immunofluorescence was possible in a continuous flow microfluorometer, making in principle, quantitation of platelet antibodies and antigens possible.

Journal

British Journal of HaematologyWiley

Published: Jun 1, 1978

References

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