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A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions

A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions Abstract. A homologous assay system for human plasma fibronectin opsonic activity is presented. The system utilizes peripheral leukocytes purified from leukocyte concentrates and a metabolizable, radiolabeled lipid emulsion which has been gelatin‐coated. The uptake of radiolabeled lipid was shown to depend on the presence of fibronectin and was stimulated by the addition of heparin. Both polymorphonuclear leukocytes (PMNs) and monocytes were active in this system. That at least a portion of the incorporated radiolabeled lipid emulsion was phagocytosed was indicated through the use of metabolic inhibitors, trypsin, and direct electron microscopic observation. This assay was applied to the analysis of donor plasma and plasma fractions. Opsonic activity levels measured with this assay system were shown to correlate (p = 0.05) with the level of fibronectin antigen in 17 samples of dialyzed normal donor plasma. Fibronectin opsonic activity was retained by outdated liquid plasma, cryo‐depleted plasma, and cryoprecipitate; however, Cohn fraction I appeared to lose considerable fibronectin opsonic activity relative to fibronectin antigen. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vox Sanguinis Wiley

A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions

Chang, Ming‐der; Chin, Sing; Horowitz, Bernard
Vox Sanguinis , Volume 48 (4) – Apr 1, 1985
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Publisher
Wiley
Copyright
© 1985 S. Karger AG, Basel
ISSN
0042-9007
eISSN
1423-0410
DOI
10.1111/j.1423-0410.1985.tb00175.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. A homologous assay system for human plasma fibronectin opsonic activity is presented. The system utilizes peripheral leukocytes purified from leukocyte concentrates and a metabolizable, radiolabeled lipid emulsion which has been gelatin‐coated. The uptake of radiolabeled lipid was shown to depend on the presence of fibronectin and was stimulated by the addition of heparin. Both polymorphonuclear leukocytes (PMNs) and monocytes were active in this system. That at least a portion of the incorporated radiolabeled lipid emulsion was phagocytosed was indicated through the use of metabolic inhibitors, trypsin, and direct electron microscopic observation. This assay was applied to the analysis of donor plasma and plasma fractions. Opsonic activity levels measured with this assay system were shown to correlate (p = 0.05) with the level of fibronectin antigen in 17 samples of dialyzed normal donor plasma. Fibronectin opsonic activity was retained by outdated liquid plasma, cryo‐depleted plasma, and cryoprecipitate; however, Cohn fraction I appeared to lose considerable fibronectin opsonic activity relative to fibronectin antigen.

Journal

Vox Sanguinis – Wiley

Published: Apr 1, 1985

References

  • Isolation of a collagen‐binding fragment from fibronectin and cold‐insoluble globulin
    Balian, Balian; Click, Click; Crouch, Crouch; Davidson, Davidson; Bronstein, Bronstein
  • Receptors for cold‐insoluble globulin (plasma fibronectin) on human monocytes
    Bevilacqua, Bevilacqua; Amrani, Amrani; Mosesson, Mosesson; Bianco, Bianco
  • Opsonic fibronectin after trauma and particle ingestion determined by a peritoneal macrophage monolayer assay
    Blumenstock, Blumenstock; Saba, Saba; Roccario, Roccario; Cho, Cho; Kaplan, Kaplan
  • Agglutination assay for human opsonic factor using gelatin‐coated latex particles
    Check, Check; Wolfmann, Wolfmann; Coley, Coley; Hunter, Hunter
  • The development of a lipid emulsion for the measurement of reticuloendothelial function
    DiLuzio, DiLuzio; Riggi, Riggi
  • Surgery‐induced alterations in plasma recognition factor activity in normal renal donors and renal recipients
    DiLuzio, DiLuzio; Lindsey, Lindsey
  • Cold insoluble globulin‐enhanced phagocytosis of gelatinized targets by macrophage monolayers. A model system
    Doran, Doran; Mansberger, Mansberger; Reese, Reese
  • Cold insoluble globulin and heparin interaction in phagocytosis by macrophage monolayers. Mechanism of heparin enhancement
    Doran, Doran; Mansberger, Mansberger; Edmondson, Edmondson; Reese, Reese
  • Binding of soluble form of fibroblast surface protein, fibronectin, to collagen
    Engvall, Engvall; Ruoslahti, Ruoslahti
  • Fibronectin in healing rabbit corneal wounds
    Fujikawa, Fujikawa; Foster, Foster; Harriet, Harriet; Lanigan, Lanigan; Colvin, Colvin
  • Fibroblast receptor for cell‐substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin)
    Grinnell, Grinnell
  • Distribution of fibronectin during wound healing in vivo
    Grinnell, Grinnell; Billingham, Billingham; Burgess, Burgess
  • Fibronectin‐mediated uptake of gelatin‐coated latex particles by peritoneal macrophages
    Gudewicz, Gudewicz; Molnar, Molnar; Lai, Lai; Beezhold, Beezhold; Siefring, Siefring; Credo, Credo; Lorand, Lorand
  • Domain structure of the carboxyl‐terminal half of human plasma fibronectin
    Hayashi, Hayashi; Yamada, Yamada
  • Preparation of antihemophilic factor and fibronectin from human plasma cryoprecipitate
    Horowitz, Horowitz; Lippin, Lippin; Chang, Chang; Shulman, Shulman; Vandersande, Vandersande; Stryker, Stryker; Woods, Woods
  • A rapid micromethod for the determination of nitrogen and phosphate in biological material
    Jaenicke, Jaenicke
  • Fibronectin (cold‐insoluble globulin). VI. Influence of heparin and hyaluronic acid on the binding of native collagen
    Jilek, Jilek; Hormann, Hormann
  • Fibronectin (cold‐insoluble globulin), Mediation of fibrin‐monomer binding to macrophage
    Jilek, Jilek; Hormann, Hormann
  • Electron microscopy study of fibronectin structure
    Koteliansky, Koteliansky; Benjanian, Benjanian; Smirnov, Smirnov
  • Fibronectin as a cofactor necessary for optimal granulocyte phagocytosis of Staphylococcus aureus.
    Lanser, Lanser; Saba, Saba
  • Electroimmunoassay
    Laurell, Laurell
  • Degradation of fibronectin by human leukocyte elastase. Release of biologically active fragments
    McDonald, McDonald; Kelley, Kelley
  • The structure and biologic activities of plasma fibronectin
    Mosesson, Mosesson; Amrani, Amrani
  • Binding and factor XIIIa‐mediated cross‐linking of a 27‐kilodalton fragment of fibronectin to Staphylococcus aureus.
    Mosher, Mosher; Proctor, Proctor
  • Cross‐linking of collagen and fibronectin by factor XIIIa
    Mosher, Mosher; Schad, Schad; Vann, Vann
  • Metabolic patterns in three types of phagocytizing cells
    Oren, Oren; Franham, Franham; Saito, Saito; Milofsky, Milofsky; Karnovsky, Karnovsky
  • Location of the cell‐attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule
    Pierschbacher, Pierschbacher; Hayman, Hayman; Ruoslahti, Ruoslahti
  • Reduced plasma fibronectin in patient with sepsis and shock
    Pott, Pott; Lohmann, Lohmann; Zundorf, Zundorf; Gerlack, Gerlack
  • Fibronectin mediates attachment of Staphylococcus aureus to human neutrophils
    Proctor, Proctor; Prendergast, Prendergast; Mosher, Mosher
  • A polymorphonuclear leukocyte monolayer system for studies of phagocytosis
    Raynor, Raynor; Doran, Doran; Reese, Reese
  • Kupffer cell phagocytosis and metabolism of a variety of particles as a function of opsonization
    Saba, Saba; DiLuzio, DiLuzio
  • Reticuloendothelial systemic host defense after surgery and traumatic shock
    Saba, Saba
  • Reticuloendothelial systemic response to operative trauma as influenced by cryoprecipitate or cold‐insoluble globulin therapy
    Saba, Saba; Cho, Cho
  • Identification of two fibrin‐binding domains in plasma fibronectin and unequal distribution of these domains in two different subunits: a preliminary note
    Sekiguchi, Sekiguchi; Hakomori, Hakomori
  • Human serum fibronectin mediates attachment of nonvirulent Streptococcus pyrogenes to human neutrophils
    Simpson, Simpson; Pinals, Pinals; Hasty, Hasty; Mason, Mason
  • Interactions among heparin, cold‐insoluble globulin, and fibrinogen in formation of the heparin‐precipitable fraction of plasma
    Stathakis, Stathakis; Mosesson, Mosesson
  • Cryoprecipitation of fibrin‐fibrinogen complexes induced by the cold‐insoluble globulin of plasma
    Stathakis, Stathakis; Mosesson, Mosesson; Chen, Chen; Galanakis, Galanakis
  • Quantitative studies of phagocytosis by polymorphonuclear leukocytes: use of emulsions to measure the initial rate of phagocytosis
    Stossel, Stossel; Mason, Mason; Hartwig, Hartwig; Vaughan, Vaughan
  • Phagocytosis of gelatinlatex particles by a marine macrophage line is dependant on fibronectin and heparin
    Water, Water; Schroeder, Schroeder; Crenshaw, Crenshaw; Hynes, Hynes
  • Tumor‐promoting phobol esters stimulate C 3 b and C 3 b' receptor‐mediated phagocytosis in cultured human monocytes
    Wright, Wright; Silverstein, Silverstein
  • Fibronectins–adhesive glycoproteins of cell surface and blood
    Yamada, Yamada; Olden, Olden
  • Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments
    Yamada, Yamada; Kennedy, Kennedy; Kimata, Kimata; Pratt, Pratt

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