A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions

A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions Abstract. A homologous assay system for human plasma fibronectin opsonic activity is presented. The system utilizes peripheral leukocytes purified from leukocyte concentrates and a metabolizable, radiolabeled lipid emulsion which has been gelatin‐coated. The uptake of radiolabeled lipid was shown to depend on the presence of fibronectin and was stimulated by the addition of heparin. Both polymorphonuclear leukocytes (PMNs) and monocytes were active in this system. That at least a portion of the incorporated radiolabeled lipid emulsion was phagocytosed was indicated through the use of metabolic inhibitors, trypsin, and direct electron microscopic observation. This assay was applied to the analysis of donor plasma and plasma fractions. Opsonic activity levels measured with this assay system were shown to correlate (p = 0.05) with the level of fibronectin antigen in 17 samples of dialyzed normal donor plasma. Fibronectin opsonic activity was retained by outdated liquid plasma, cryo‐depleted plasma, and cryoprecipitate; however, Cohn fraction I appeared to lose considerable fibronectin opsonic activity relative to fibronectin antigen. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vox Sanguinis Wiley

A New Assay for Fibronectin Opsonic Activity and Its Application to Plasma and Plasma Fractions

Vox Sanguinis, Volume 48 (4) – Apr 1, 1985

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Publisher
Wiley
Copyright
© 1985 S. Karger AG, Basel
ISSN
0042-9007
eISSN
1423-0410
DOI
10.1111/j.1423-0410.1985.tb00175.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. A homologous assay system for human plasma fibronectin opsonic activity is presented. The system utilizes peripheral leukocytes purified from leukocyte concentrates and a metabolizable, radiolabeled lipid emulsion which has been gelatin‐coated. The uptake of radiolabeled lipid was shown to depend on the presence of fibronectin and was stimulated by the addition of heparin. Both polymorphonuclear leukocytes (PMNs) and monocytes were active in this system. That at least a portion of the incorporated radiolabeled lipid emulsion was phagocytosed was indicated through the use of metabolic inhibitors, trypsin, and direct electron microscopic observation. This assay was applied to the analysis of donor plasma and plasma fractions. Opsonic activity levels measured with this assay system were shown to correlate (p = 0.05) with the level of fibronectin antigen in 17 samples of dialyzed normal donor plasma. Fibronectin opsonic activity was retained by outdated liquid plasma, cryo‐depleted plasma, and cryoprecipitate; however, Cohn fraction I appeared to lose considerable fibronectin opsonic activity relative to fibronectin antigen.

Journal

Vox SanguinisWiley

Published: Apr 1, 1985

References

  • Receptors for cold‐insoluble globulin (plasma fibronectin) on human monocytes
    Bevilacqua, Bevilacqua; Amrani, Amrani; Mosesson, Mosesson; Bianco, Bianco
  • Binding of soluble form of fibroblast surface protein, fibronectin, to collagen
    Engvall, Engvall; Ruoslahti, Ruoslahti
  • Fibroblast receptor for cell‐substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin)
    Grinnell, Grinnell
  • Fibronectin‐mediated uptake of gelatin‐coated latex particles by peritoneal macrophages
    Gudewicz, Gudewicz; Molnar, Molnar; Lai, Lai; Beezhold, Beezhold; Siefring, Siefring; Credo, Credo; Lorand, Lorand
  • Fibronectin (cold‐insoluble globulin). VI. Influence of heparin and hyaluronic acid on the binding of native collagen
    Jilek, Jilek; Hormann, Hormann
  • Metabolic patterns in three types of phagocytizing cells
    Oren, Oren; Franham, Franham; Saito, Saito; Milofsky, Milofsky; Karnovsky, Karnovsky

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