Background We previously developed a method for counting CD34+ cells in unlysed whole blood. This method was applied to normal human bone marrow, peripheral blood after mobilization of progenitor cells, leukapheresis products, and cord blood and was validated with two different lyse‐no wash methods. However, the main advantage that we described, erythrocyte discrimination using nucleic acid staining, was also the main restriction because additional markers for the immunologic characterization of CD34+ cells cannot be included. Methods We used SYTO‐13 and fluorescein isothiocyanate (FITC)‐CD45 staining (FL1) instead of SYTO‐13 and phycoerythrin (PE)Cy5‐CD45 staining (FL3) to leave the third and fourth fluorescence parameters available for further characterization of CD34+ cells. The new method was validated by applying it to cord blood samples (n = 20). Results FITC‐CD45 antibody gave a 1.7‐fold increase in mean fluorescence intensity over SYTO‐13 alone. From absolute counts (CD34+ cells per microliter), we plotted the differences between the methods against their mean, showing that differences fell into acceptable ranges. Conclusions No‐lyse procedures may represent an advance for cell immunophenotyping and it could be applied to the measurement of additional markers. Cytometry (Clin. Cytometry) 50:249–253, 2002. © 2002 Wiley‐Liss, Inc.
Cytometry Part A – Wiley
Published: Mar 15, 2003
Keywords: ; ; ; ; ; ;
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera