A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

A geminivirus replication protein interacts with the retinoblastoma protein through a novel... Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homo logues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1–pRBR interactions. Plants infected with the E‐N140 mutant, which is wild‐type for pRBR binding, developed wild‐type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1–pRBR interactions during geminivirus infection of plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
DOI
10.1093/emboj/19.13.3485
Publisher site
See Article on Publisher Site

Abstract

Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homo logues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1–pRBR interactions. Plants infected with the E‐N140 mutant, which is wild‐type for pRBR binding, developed wild‐type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1–pRBR interactions during geminivirus infection of plants.

Journal

The EMBO JournalWiley

Published: Mar 3, 2000

Keywords: ; ; ; ;

References

  • A conserved family of WD‐40 proteins binds to the retinoblastoma protein in both plants and animals
    Ach, RA; Taranto, P; Gruissem, W
  • Geminivirus replication origins have a modular organization
    Fontes, EPB; Gladfelter, HJ; Schaffer, RL; Petty, ITD; Hanley‐Bowdoin, L
  • DNA replication and cell cycle in plants: learning from geminiviruses
    Gutierrez, C
  • Cdc2A expression in Arabidopsis is linked with competence for cell division
    Hemerly, AS; Ferreira, P; Engler, JD; Van Montagu, M; Engler, G; Inze, D
  • Prediction of functional regions of the maize streak virus replication‐associated proteins by protein–protein interaction analysis
    Horvath, GV; Pettko‐Szandtner, A; Nikovics, K; Bilgin, M; Boulton, M; Davies, JW; Gutierrez, C; Dudits, D
  • A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells
    Nagar, S; Pedersen, TJ; Carrick, K; Hanley‐Bowdoin, L; Robertson, D
  • Tobacco retinoblastoma‐related protein phosphorylated by a distinct cyclin‐dependent kinase complex with Cdc2/cyclin D in vitro
    Nakagami, H; Sekine, M; Murakami, H; Shinmyo, A
  • A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif
    Soni, R; Carmichael, JP; Shah, ZH; Murray, JAH
  • Cell division in plants
    Staiger, C; Doonan, J
  • Differential expression of genes for cyclin‐dependent protein kinases in rice plants
    Umeda, M; Umeda‐Hara, C; Yamaguchi, M; Hashimoto, J; Uchimiya, H

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