Abstract: Metabolism of (1‐14C)glucose and (6‐14C)glucose was measured in sympathetic ganglia excised from chicken embryos 12–16 days old and in primary cultures of neurons or nonneurons prepared from these ganglia. Some metabolic rates tended to change with the tissue/medium ratio, so this variable had to be controlled. Less C‐6 than C‐1 of glucose was put out in CO2 by all three types of preparations, indicating operation of the hexosemonophosphate shunt. The C‐6/C‐1 ratio was greater for the neuronal cultures and for intact ganglia than for the nonneuronal cultures. The C‐6/C‐1 ratio for the neurons increased with the amount of tissue added to a given volume of incubation medium, in agreement with previous experiments on embryonic dorsal root ganglia (Larrabee, 1978). Per unit of protein, the output of C‐1 of glucose in CO2 was higher in both the neuronal and the nonneuronal cultures than in intact ganglia, whereas that of C‐6 was higher in the neuronal cultures and lower in the nonneuronal ones than in the ganglia. The rates of release in lactate of C‐1 and C‐6 of glucose were 3–5 times higher from both types of cultures than from intact ganglia. The average rates of incorporation of C‐1 and C‐6 of glucose into tissue constituents were lower in the cultures than in intact ganglia, significantly so for incorporation of C‐6 in the nonneuronal cultures.
Journal of Neurochemistry – Wiley
Published: Jan 1, 1982
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