( 14 C)Glucose Metabolism in Sympathetic Ganglia of Chicken Embryos and in Primary Cultures of Neurons and of Other Cells from These Ganglia

( 14 C)Glucose Metabolism in Sympathetic Ganglia of Chicken Embryos and in Primary Cultures of... Abstract: Metabolism of (1‐14C)glucose and (6‐14C)glucose was measured in sympathetic ganglia excised from chicken embryos 12–16 days old and in primary cultures of neurons or nonneurons prepared from these ganglia. Some metabolic rates tended to change with the tissue/medium ratio, so this variable had to be controlled. Less C‐6 than C‐1 of glucose was put out in CO2 by all three types of preparations, indicating operation of the hexosemonophosphate shunt. The C‐6/C‐1 ratio was greater for the neuronal cultures and for intact ganglia than for the nonneuronal cultures. The C‐6/C‐1 ratio for the neurons increased with the amount of tissue added to a given volume of incubation medium, in agreement with previous experiments on embryonic dorsal root ganglia (Larrabee, 1978). Per unit of protein, the output of C‐1 of glucose in CO2 was higher in both the neuronal and the nonneuronal cultures than in intact ganglia, whereas that of C‐6 was higher in the neuronal cultures and lower in the nonneuronal ones than in the ganglia. The rates of release in lactate of C‐1 and C‐6 of glucose were 3–5 times higher from both types of cultures than from intact ganglia. The average rates of incorporation of C‐1 and C‐6 of glucose into tissue constituents were lower in the cultures than in intact ganglia, significantly so for incorporation of C‐6 in the nonneuronal cultures. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

( 14 C)Glucose Metabolism in Sympathetic Ganglia of Chicken Embryos and in Primary Cultures of Neurons and of Other Cells from These Ganglia

Journal of Neurochemistry, Volume 38 (1) – Jan 1, 1982

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Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1982.tb10874.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Metabolism of (1‐14C)glucose and (6‐14C)glucose was measured in sympathetic ganglia excised from chicken embryos 12–16 days old and in primary cultures of neurons or nonneurons prepared from these ganglia. Some metabolic rates tended to change with the tissue/medium ratio, so this variable had to be controlled. Less C‐6 than C‐1 of glucose was put out in CO2 by all three types of preparations, indicating operation of the hexosemonophosphate shunt. The C‐6/C‐1 ratio was greater for the neuronal cultures and for intact ganglia than for the nonneuronal cultures. The C‐6/C‐1 ratio for the neurons increased with the amount of tissue added to a given volume of incubation medium, in agreement with previous experiments on embryonic dorsal root ganglia (Larrabee, 1978). Per unit of protein, the output of C‐1 of glucose in CO2 was higher in both the neuronal and the nonneuronal cultures than in intact ganglia, whereas that of C‐6 was higher in the neuronal cultures and lower in the nonneuronal ones than in the ganglia. The rates of release in lactate of C‐1 and C‐6 of glucose were 3–5 times higher from both types of cultures than from intact ganglia. The average rates of incorporation of C‐1 and C‐6 of glucose into tissue constituents were lower in the cultures than in intact ganglia, significantly so for incorporation of C‐6 in the nonneuronal cultures.

Journal

Journal of NeurochemistryWiley

Published: Jan 1, 1982

References

  • Metabolic partitioning of carbon from glucose by a mammalian sympathetic ganglion
    Horowicz, Horowicz; Larrabee, Larrabee
  • A new mathematical approach to the metabolism of ( 14 C)glucose, with applications to sensory ganglia of chicken embryos
    Larrabee, Larrabee
  • Addendum to a new mathematical approach to metabolism of ( 14 C)glucose: The pyruvate carboxylase reaction
    Larrabee, Larrabee
  • Metabolic disposition of glucose carbon by sensory ganglia of 15‐day‐old chicken embryos, with new dynamic models of carbohydrate metabolism
    Larrabee, Larrabee

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