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Urea inhibits Na-K-2Cl cotransport in medullary thick ascending limb cells

Urea inhibits Na-K-2Cl cotransport in medullary thick ascending limb cells Kaji, Deepak M., J. Diaz, and John C. Parker. hibits Na-K-2Cl medullary thick ascendg limb . Am. J. PhysioZ. 272 (CelZ Physiol. 41): C615-C621, 1997.We examed the effect of physiological concentrations of (100-500 mM) on Na-K-2Cl cultured from mouse medullary thick ascendg limb (mTAL). acutely hibited bumetanide-sensitive K flux mTAL a concentration-dependent fashion, with a statistically significant hibition (19%) at 100 mM and 86% hibition at 500 mM. The effect of was entirely reversible and was blocked by prior treatment with okadaic acid, a phosphatase hibitor, suggestg that exerts its action upstream of the phosphorylation-dephosphorylation step. Cell volume was unchanged the presence of 500 mM . The number of [3H]bumetanide bdg sites, a measure of the number of functiong er sites, was decreased the presence of , and the decrease bumetanide bdg was proportional to the decrease bumetanidesensitive K flux. also stimulated the Ba-sensitive swellg-activated K efflux from mTAL . Thus , concentrations that prevail the renal medulla, alters ion mTAL . anion-dependent phosphatase cation ; calycul; kase; prote tions (100-500 mM) alters volume-sensitive pathways erythrocytes from a variety of mammalian species (3, 10, 12, 16). However, the effect of on volume-sensitive nucleated has not been reported to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Urea inhibits Na-K-2Cl cotransport in medullary thick ascending limb cells

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Publisher
The American Physiological Society
Copyright
Copyright © 1997 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

Kaji, Deepak M., J. Diaz, and John C. Parker. hibits Na-K-2Cl medullary thick ascendg limb . Am. J. PhysioZ. 272 (CelZ Physiol. 41): C615-C621, 1997.We examed the effect of physiological concentrations of (100-500 mM) on Na-K-2Cl cultured from mouse medullary thick ascendg limb (mTAL). acutely hibited bumetanide-sensitive K flux mTAL a concentration-dependent fashion, with a statistically significant hibition (19%) at 100 mM and 86% hibition at 500 mM. The effect of was entirely reversible and was blocked by prior treatment with okadaic acid, a phosphatase hibitor, suggestg that exerts its action upstream of the phosphorylation-dephosphorylation step. Cell volume was unchanged the presence of 500 mM . The number of [3H]bumetanide bdg sites, a measure of the number of functiong er sites, was decreased the presence of , and the decrease bumetanide bdg was proportional to the decrease bumetanidesensitive K flux. also stimulated the Ba-sensitive swellg-activated K efflux from mTAL . Thus , concentrations that prevail the renal medulla, alters ion mTAL . anion-dependent phosphatase cation ; calycul; kase; prote tions (100-500 mM) alters volume-sensitive pathways erythrocytes from a variety of mammalian species (3, 10, 12, 16). However, the effect of on volume-sensitive nucleated has not been reported to

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Feb 1, 1997

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