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Upregulation of inositol transport mediates inositol accumulation in hyperosmolar brain cells

Upregulation of inositol transport mediates inositol accumulation in hyperosmolar brain cells STRANGE, KEV, REBECCA MORRISON, CHARLES W. HEILIG, SUSAN DIPIETRO, AND STEVEN R. GULLANS. Upregulation of ositol transport mediates ositol hyperosmoZar ceLZs.Am. J. Physiol. 260 (Cell Physiol. 29): C784C790, 1991.-Attempts to understand regulation have been greatly hampered by the structural complexity of the mammalian central nervous system, dicatg a need for the vestigation of cultured cell les whose behavior reflects that observed situ. We demonstrate here that rat C6 glioma exhibit a pattern of hyperosmolar regulation qualitatively similar to that of the tact . Chronic (2-6 days) acclimation of C6 to high NaCl media (440 or 590 mosM) resulted a 46-133 mM crease cellular ositol, a known major osmolyte. C6 exposed acutely to 440 mosM medium shrank abruptly and then underwent a complete crease (RVI) with 4 h. ositol levels began to crease after 10 h of hyperosmolar stress and reached maximal values by 24 h, suggestg that RVI is itially mediated by organic ion uptake. [3H]ositol uptake measurements revealed a sevenfold stimulation of phloriz-hibitable ositol transport hyperosmotic . The enhancement of ositol transport paralleled the rise cellular ositol content. Phloriz reduced ositol hyperosmolar by 44%. Our studies provide the first demonstration of RVI and organic osmolyte a cultured http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Upregulation of inositol transport mediates inositol accumulation in hyperosmolar brain cells

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Publisher
The American Physiological Society
Copyright
Copyright © 1991 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
See Article on Publisher Site

Abstract

STRANGE, KEV, REBECCA MORRISON, CHARLES W. HEILIG, SUSAN DIPIETRO, AND STEVEN R. GULLANS. Upregulation of ositol transport mediates ositol hyperosmoZar ceLZs.Am. J. Physiol. 260 (Cell Physiol. 29): C784C790, 1991.-Attempts to understand regulation have been greatly hampered by the structural complexity of the mammalian central nervous system, dicatg a need for the vestigation of cultured cell les whose behavior reflects that observed situ. We demonstrate here that rat C6 glioma exhibit a pattern of hyperosmolar regulation qualitatively similar to that of the tact . Chronic (2-6 days) acclimation of C6 to high NaCl media (440 or 590 mosM) resulted a 46-133 mM crease cellular ositol, a known major osmolyte. C6 exposed acutely to 440 mosM medium shrank abruptly and then underwent a complete crease (RVI) with 4 h. ositol levels began to crease after 10 h of hyperosmolar stress and reached maximal values by 24 h, suggestg that RVI is itially mediated by organic ion uptake. [3H]ositol uptake measurements revealed a sevenfold stimulation of phloriz-hibitable ositol transport hyperosmotic . The enhancement of ositol transport paralleled the rise cellular ositol content. Phloriz reduced ositol hyperosmolar by 44%. Our studies provide the first demonstration of RVI and organic osmolyte a cultured

Journal

AJP - Cell PhysiologyThe American Physiological Society

Published: Apr 1, 1991

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