nitric cell line STEPHEN C. LINN, PETER J. MORELLI, IRIS EDRY, SARAH E. COTTONGIM, CSABA SZAB& AND ANDREW L. SALZMAN Division of Critical Care Medicine, Childrenâs Hospital Medical Center, Cincinnati, Linn, Stephen C., Peter J. Morelli, Iris Edry, Sarah E. Cottongim, Csaba Szab6, andAndrew L. Salzman. Transcriptional of nitric oxide in an intestinal epithelial cell line. Am. J. PhysioZ. 272 (Gastrointest. Liver Physiol. 35): Gl499-G1508, 1997. The form of nitric oxide (iS) is expressed during inflammation of the intestine and may contribute to tissue injury. We have examined iS transcriptional in DLD-1 cells, a intestinal epithelial line that produces large amounts of nitric oxide and iS mRNA in response to a combination of the proinflammatory cytokines interleukin-1 p (IL-l p) and interferon-y (IFN-y). Levels of iS mRNA are extremely low in unstimulated DLD-1 cells but increase dramatically after cytokine treatment. Nuclear run-on analyses demonstrated that transcriptional activation, which accounts for a portion of this increase, is dependent on both IL-lp and IFN-y and requires de vo protein synthesis. Transfection of DLD-1 cells with reporter constructs containing deletions of the iS promoter showed that sequences located between 8.7 and 10.7 kb upstream of the transcription initiation site are necessary
AJP - Gastrointestinal and Liver Physiology – The American Physiological Society
Published: Jun 1, 1997
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