METHODS Adult (350-450 g) male Sprague-Dawleyrats (Harlan Sprague-Dawley, Indianapolis, IN) were anesthetized with sodium thiopental (100 mg/kg, 0.1 ml/100 g ip) (Abbott Laboratories, N. Chicago, IL). The trachea wasintubated, and the right femoral artery was cannulated to measurearterial blood pressure. Data from an animal were usedonly if the meanarterial pressurewas >95 mmHg and was essentiallyconstant the 2to 3-h data-collection period. A midline abdominal incision was used to exposethe small intestine, and the ileum wasidentified using the appendix asan anatomic reference.A lo- to 12-cm loop of ileum was exteriorized into a heated bath of physiological saline (Normasol-R, Abbott Laboratories). Two small incisions were made with a thermal cautery -4 cm apart alongthe antimesentericborder of the bowel. Food or mucus was flushed out of the bowel lumen through the incisions. Three sutures were tied to the antimesenteric border of the bowel at - l-cm intervals, and the bowel H219 0363-6135/93 $2.00 Copyright 0 1993 the American Physiological Society H220 EDRF RESPONSES was draped over a translucent pedestal in the heated (37Â°C) bath. The closed-bowel preparation was used to prevent exposure of the intestinal lumen to D-ghCOSe solutions because Dglucose absorption by the mucosa causes vasodilation to support the absorptive process
AJP - Heart and Circulatory Physiology – The American Physiological Society
Published: Jul 1, 1993
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