Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Short-term effect of aldosterone on Na-K-ATPase in single nephron segments

Short-term effect of aldosterone on Na-K-ATPase in single nephron segments Abstract One of the theories proposed to explain the action of mineralocorticoids on target tissues attributes their effect on cation transport to stimulation of the “sodium pump.” To test this hypothesis we evaluated the short-term effect of aldosterone on Na-K-ATPase in discrete nephron segments microdissected from kidneys of adrenalectomized animals. Na-K-ATPase activity measured in proximal and distal nephron segments from adrenalectomized as well as intact mice remained unchanged 1 or 3 h after a single injection of D-aldosterone (10 micrograms/100 g body wt i.p.). Similarly, no effect of aldosterone could be demonstrated in experiments in vitro, in which tubules from adrenalectomized mice and rabbits were incubated (90 min, 37 degrees C) in the presence of physiologic or pharmacologic concentrations of the hormone (3.10(-10) to 3.10(-6) M). These studies did not show an effect of aldosterone on isolated tubule Na-K-ATPase within the time interval in which this hormone alters tubular cation transport and therefore do not support the “pump theory” of mineralocorticoid action. Copyright © 1981 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Renal Physiology The American Physiological Society

Short-term effect of aldosterone on Na-K-ATPase in single nephron segments

AJP - Renal Physiology , Volume 241 (3): F273 – Sep 1, 1981

Loading next page...
 
/lp/the-american-physiological-society/short-term-effect-of-aldosterone-on-na-k-atpase-in-single-nephron-xAYIazUsMB

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
The American Physiological Society
Copyright
Copyright © 1981 the American Physiological Society
ISSN
0363-6127
eISSN
1522-1466
Publisher site
See Article on Publisher Site

Abstract

Abstract One of the theories proposed to explain the action of mineralocorticoids on target tissues attributes their effect on cation transport to stimulation of the “sodium pump.” To test this hypothesis we evaluated the short-term effect of aldosterone on Na-K-ATPase in discrete nephron segments microdissected from kidneys of adrenalectomized animals. Na-K-ATPase activity measured in proximal and distal nephron segments from adrenalectomized as well as intact mice remained unchanged 1 or 3 h after a single injection of D-aldosterone (10 micrograms/100 g body wt i.p.). Similarly, no effect of aldosterone could be demonstrated in experiments in vitro, in which tubules from adrenalectomized mice and rabbits were incubated (90 min, 37 degrees C) in the presence of physiologic or pharmacologic concentrations of the hormone (3.10(-10) to 3.10(-6) M). These studies did not show an effect of aldosterone on isolated tubule Na-K-ATPase within the time interval in which this hormone alters tubular cation transport and therefore do not support the “pump theory” of mineralocorticoid action. Copyright © 1981 the American Physiological Society

Journal

AJP - Renal PhysiologyThe American Physiological Society

Published: Sep 1, 1981

There are no references for this article.