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Sarcolemmal enzymes and Na+ -Ca2+ exchange in hypoxic, ischemic, and reperfused rat hearts

Sarcolemmal enzymes and Na+ -Ca2+ exchange in hypoxic, ischemic, and reperfused rat hearts JENNIFER S. ELZ, WINIFRED G. NAYLER of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia MICHAEL J., JENNIFER S. ELZ, WINIFRED G. Sacrolemmal enzymes Na’-Ca2’ exchange in hypoxic, ischemic, reperfused rat hearts. Am. J. Physiol. 247 DALY, NAYLER. (Heart Circ. Physiol. 16): H237-H243, 1984.-Isolated spontaneously beating rat hearts were perfused in the Langendorff mode divided into four groups, i.e., control (aerobic), hypoxic (95% N,-5% C02), ischemic, ischemic reperfused. After a total of 90 min of perfusion, the isolated was enzymatically characterized. Ouabain-sensitive Na+-K’ATPase wasinhibited in all three experimental groups,whereas K+-stimulated phosphataseactivity was decreased only in the ischemic reperfused groups compared with control. 5’Nucleotidase activity was inhibited (P < 0.05) only in the ischemicgroup. Mg2’-ATPase activity was not different from control. PassiveCa2+uptake Ca2’ efflux were not significantly altered by any of the interventions. Na+-Ca2+ exchange rate, but not capacity, wasdecreased 32-42%) in the ische(by mic group but this waspartially reversedon reperfusion. These results suggestthat changessecondary to lack of flow rather METHODS than O2play a major role in the etiology of ischemicdamage to Adult male Sprague-Dawley rats (200-275 g) were the membrane that a 15-min period of reperfusion after 60 anesthetized with a diethylether-O2 mixture heparinmin of ischemiadoesnot exacerbate http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

Sarcolemmal enzymes and Na+ -Ca2+ exchange in hypoxic, ischemic, and reperfused rat hearts

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Publisher
The American Physiological Society
Copyright
Copyright © 1984 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
Publisher site
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Abstract

JENNIFER S. ELZ, WINIFRED G. NAYLER of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia MICHAEL J., JENNIFER S. ELZ, WINIFRED G. Sacrolemmal enzymes Na’-Ca2’ exchange in hypoxic, ischemic, reperfused rat hearts. Am. J. Physiol. 247 DALY, NAYLER. (Heart Circ. Physiol. 16): H237-H243, 1984.-Isolated spontaneously beating rat hearts were perfused in the Langendorff mode divided into four groups, i.e., control (aerobic), hypoxic (95% N,-5% C02), ischemic, ischemic reperfused. After a total of 90 min of perfusion, the isolated was enzymatically characterized. Ouabain-sensitive Na+-K’ATPase wasinhibited in all three experimental groups,whereas K+-stimulated phosphataseactivity was decreased only in the ischemic reperfused groups compared with control. 5’Nucleotidase activity was inhibited (P < 0.05) only in the ischemicgroup. Mg2’-ATPase activity was not different from control. PassiveCa2+uptake Ca2’ efflux were not significantly altered by any of the interventions. Na+-Ca2+ exchange rate, but not capacity, wasdecreased 32-42%) in the ische(by mic group but this waspartially reversedon reperfusion. These results suggestthat changessecondary to lack of flow rather METHODS than O2play a major role in the etiology of ischemicdamage to Adult male Sprague-Dawley rats (200-275 g) were the membrane that a 15-min period of reperfusion after 60 anesthetized with a diethylether-O2 mixture heparinmin of ischemiadoesnot exacerbate

Journal

AJP - Heart and Circulatory PhysiologyThe American Physiological Society

Published: Aug 1, 1984

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