Role of nitroxidergic nerve in dog retinal arterioles in vivo and arteries in vitro

Role of nitroxidergic nerve in dog retinal arterioles in vivo and arteries in vitro METHODS Studies vitro. Mongrel dogs of either sex, weighg 7-15 kg, were anesthetized with travenous jections of pentobarbital sodium (30 mg/kg) killed by bleedg from the carotid arteries. The eyeballs attached with optic s extraocular tissues were isolated from the orbital cavities. Extraocular muscles were removed, the retal central arteries (0.3-0.4 mm OD) just before enterg to the eyeball were carefully isolated. The arteries were cut to helical strips of - 10 mm long, with care to preserve the endothelium. The specimens were vertically fixed between hooks a muscle bath contag the modified Rger-Locke solution that was mataed at 37 t_ 0.3”C aerated with a mixture of 95% 02- 5% COa. The hooks anchorg the upper end of the strips were connected to the lever of a force-displacement transducer. The restg tension was adjusted to 0.7 g, which is optimal of ducg the maximal contraction. The composition of the bathg medium was as follows ( mM): 120 NaCl, 5.4 KCl, 2.2 CaC12, 1.0 MgC12, 25.0 NaHC03, 5.6 dextrose. The pH of the solution was 7.35-7.42. Before the start of experiments, all of the strips were allowed to equilibrate for 60-90 m the bathg media, durg which time the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

Role of nitroxidergic nerve in dog retinal arterioles in vivo and arteries in vitro

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
Publisher site
See Article on Publisher Site

Abstract

METHODS Studies vitro. Mongrel dogs of either sex, weighg 7-15 kg, were anesthetized with travenous jections of pentobarbital sodium (30 mg/kg) killed by bleedg from the carotid arteries. The eyeballs attached with optic s extraocular tissues were isolated from the orbital cavities. Extraocular muscles were removed, the retal central arteries (0.3-0.4 mm OD) just before enterg to the eyeball were carefully isolated. The arteries were cut to helical strips of - 10 mm long, with care to preserve the endothelium. The specimens were vertically fixed between hooks a muscle bath contag the modified Rger-Locke solution that was mataed at 37 t_ 0.3”C aerated with a mixture of 95% 02- 5% COa. The hooks anchorg the upper end of the strips were connected to the lever of a force-displacement transducer. The restg tension was adjusted to 0.7 g, which is optimal of ducg the maximal contraction. The composition of the bathg medium was as follows ( mM): 120 NaCl, 5.4 KCl, 2.2 CaC12, 1.0 MgC12, 25.0 NaHC03, 5.6 dextrose. The pH of the solution was 7.35-7.42. Before the start of experiments, all of the strips were allowed to equilibrate for 60-90 m the bathg media, durg which time the

Journal

AJP - Heart and Circulatory PhysiologyThe American Physiological Society

Published: May 1, 1994

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