Access the full text.
Sign up today, get DeepDyve free for 14 days.
Ulrich Hofmann, C. Rotsch, W. Parak, M. Radmacher (1997)
Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope.Journal of structural biology, 119 2
L. Formigli, E. Meacci, C. Sassoli, F. Chellini, R. Giannini, F. Quercioli, B. Tiribilli, R. Squecco, P. Bruni, F. Francini, S. Zecchi-Orlandini (2005)
Sphingosine 1-phosphate induces cytoskeletal reorganization in C2C12 myoblasts: physiological relevance for stress fibres in the modulation of ion current through stretch-activated channelsJournal of Cell Science, 118
I. Horcas, R. Fernandez, J. Gómez‐Rodríguez, J. Colchero, J. Gómez‐Herrero, A. Baró (2007)
WSXM: a software for scanning probe microscopy and a tool for nanotechnology.The Review of scientific instruments, 78 1
A. Staruschenko, Y. Negulyaev, E. Morachevskaya (2005)
Actin cytoskeleton disassembly affects conductive properties of stretch-activated cation channels in leukaemia cells.Biochimica et biophysica acta, 1669 1
A. Dietrich, V. Chubanov, H. Kalwa, Benjamin Rost, T. Gudermann (2006)
Cation channels of the transient receptor potential superfamily: their role in physiological and pathophysiological processes of smooth muscle cells.Pharmacology & therapeutics, 112 3
J. Vicencio, C. Ibarra, M. Estrada, M. Chiong, Dagoberto Soto, V. Parra, G. Díaz-Araya, E. Jaimovich, S. Lavandero (2006)
Testosterone induces an intracellular calcium increase by a nongenomic mechanism in cultured rat cardiac myocytes.Endocrinology, 147 3
M. Sato, D. Théret, L. Wheeler, N. Ohshima, R. Nerem (1990)
Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties.Journal of biomechanical engineering, 112 3
A. Oberhauser, Carmelu Badilla-Fernandez, M. Carrión-Vázquez, Julio Fernandez (2002)
The mechanical hierarchies of fibronectin observed with single-molecule AFM.Journal of molecular biology, 319 2
F. Francini, C. Bencini, Claudia Piperio, R. Squecco (2001)
Separation of charge movement components in mammalian skeletal muscle fibresThe Journal of Physiology, 537
A. Bausch, Ulrike Hellerer, Markus Essler, Martin Aepfelbacher, Erich Sackmann (2001)
Rapid stiffening of integrin receptor-actin linkages in endothelial cells stimulated with thrombin: a magnetic bead microrheology study.Biophysical journal, 80 6
D. Ingber (2006)
Cellular mechanotransduction: putting all the pieces together againThe FASEB Journal, 20
F. Sachs, C. Morris (1998)
Mechanosensitive ion channels in nonspecialized cells.Reviews of physiology, biochemistry and pharmacology, 132
A. Vandebrouck, J. Sabourin, J. Rivet, H. Balghi, S. Sebille, A. Kitzis, G. Raymond, C. Cognard, N. Bourmeyster, B. Constantin (2007)
Regulation of capacitative calcium entries by α1‐syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1‐syntrophinThe FASEB Journal, 21
A. Mathur, Amy Collinsworth, W. Reichert, W. Kraus, G. Truskey (2001)
Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy.Journal of biomechanics, 34 12
J. Dai, M. Sheetz (1999)
Membrane tether formation from blebbing cells.Biophysical journal, 77 6
Z. Wu, K. Wong, M. Glogauer, R. Ellen, C. McCulloch (1999)
Regulation of stretch-activated intracellular calcium transients by actin filaments.Biochemical and biophysical research communications, 261 2
J. Allingham, Robert Smith, I. Rayment (2005)
The structural basis of blebbistatin inhibition and specificity for myosin IINature Structural &Molecular Biology, 12
L. Formigli, E. Meacci, C. Sassoli, R. Squecco, D. Nosi, F. Chellini, F. Naro, F. Francini, S. Zecchi-Orlandini (2007)
Cytoskeleton/stretch‐activated ion channel interaction regulates myogenic differentiation of skeletal myoblastsJournal of Cellular Physiology, 211
B. Martinac (2004)
Mechanosensitive ion channels: molecules of mechanotransductionJournal of Cell Science, 117
S. Mebarek, Hiba Komati, F. Naro, C. Zeiller, Monica Alvisi, M. Lagarde, A. Prigent, G. Némoz (2007)
Inhibition of de novo ceramide synthesis upregulates phospholipase D and enhances myogenic differentiationJournal of Cell Science, 120
Nia Wedhas, H. Klamut, C. Dogra, A. Srivastava, S. Mohan, Ashok Kumar (2005)
Inhibition of mechanosensitive cation channels inhibits myogenic differentiation by suppressing the expression of myogenic regulatory factors and caspase‐3 activityThe FASEB Journal, 19
N. Imbert, C. Vandebrouck, G. Duport, G. Raymond, A. Hassoni, B. Constantin, M. Cullen, C. Cognard (2001)
Calcium currents and transients in co‐cultured contracting normal and Duchenne muscular dystrophy human myotubesThe Journal of Physiology, 534
L. Limozin, A. Roth, E. Sackmann (2005)
Microviscoelastic moduli of biomimetic cell envelopes.Physical review letters, 95 17
I. Ambudkar (2006)
Ca2+ signaling microdomains:platforms for the assembly and regulation of TRPC channels.Trends in pharmacological sciences, 27 1
S. Kidoaki, Takehisa Matsuda, Kenichi Yoshikawa (2006)
Relationship between Apical Membrane Elasticity and Stress Fiber Organization in Fibroblasts Analyzed by Fluorescence and Atomic Force MicroscopyBiomechanics and Modeling in Mechanobiology, 5
R. Maroto, Albert Raso, T. Wood, A. Kurosky, B. Martinac, O. Hamill (2005)
TRPC1 forms the stretch-activated cation channel in vertebrate cellsNature Cell Biology, 7
C. Riethmüller, H. Oberleithner, M. Wilhelmi, Jonas Franz, E. Schlatter, Jens Klokkers, B. Edemir (2008)
Translocation of aquaporin-containing vesicles to the plasma membrane is facilitated by actomyosin relaxation.Biophysical journal, 94 2
E. Darling, S. Zauscher, F. Guilak (2006)
Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy.Osteoarthritis and cartilage, 14 6
P. Janmey (1998)
The cytoskeleton and cell signaling: component localization and mechanical coupling.Physiological reviews, 78 3
Jingying Zhang, W. Kraus, G. Truskey (2004)
Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells.American journal of physiology. Cell physiology, 287 2
M. Chrzanowska-Wodnicka, K. Burridge (1996)
Rho-stimulated contractility drives the formation of stress fibers and focal adhesionsThe Journal of Cell Biology, 133
C. Sassoli, M. Martinesi, R. Squecco, F. Bini, S. Zecchi, F. Francini, L. Formigli, E. Meacci (2007)
Sphingosine 1-phosphate modulates Transient Receptor Potential Channel 1 (TRCP1) in skeletal muscle cells. Relevance for Connexin43-up-regulation and myogenesis
B. Minke, Boaz Cook (2002)
TRP channel proteins and signal transduction.Physiological reviews, 82 2
A. Vinckier, G. Semenza (1998)
Measuring elasticity of biological materials by atomic force microscopyFEBS Letters, 430
T. Suchyna, F. Sachs (2007)
Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubesThe Journal of Physiology, 581
L. Formigli, E. Meacci, M. Vassalli, D. Nosi, F. Quercioli, B. Tiribilli, A. Tani, R. Squecco, F. Francini, P. Bruni, S. Orlandini (2004)
Sphingosine 1‐phosphate induces cell contraction via calcium‐independent/Rho‐dependent pathways in undifferentiated skeletal muscle cellsJournal of Cellular Physiology, 198
Yin Zhang, J. Youm, H. Sung, Sang Lee, Shin-Young Ryu, Suk-Ho Lee, W. Ho, Y. Earm (2000)
Stretch‐activated and background non‐selective cation channels in rat atrial myocytesThe Journal of Physiology, 523
Yoshihiro Yoshikawa, Toshihiro Yasuike, A. Yagi, Takenori Yamada (1999)
Transverse elasticity of myofibrils of rabbit skeletal muscle studied by atomic force microscopy.Biochemical and biophysical research communications, 256 1
I. Sneddon (1965)
The relation between load and penetration in the axisymmetric boussinesq problem for a punch of arbitrary profileInternational Journal of Engineering Science, 3
J. Kim, P. Rhee, T. Kang (2007)
Actin cytoskeletons regulate the stretch-induced increase of Ca2+ current in human gastric myocytes.Biochemical and biophysical research communications, 352 2
S. Kasas, S. Kasas, X. Wang, H. Hirling, R. Marsault, B. Huni, A. Yersin, Romano Regazzi, Gabriele Grenningloh, Beat Riederer, L. Forró, G. Dietler, Stefan Catsicas (2005)
Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly.Cell motility and the cytoskeleton, 62 2
T. Herring, C. Cohan, E. Welnhofer, L. Mills, C. Morris (1999)
F-actin at Newly Invaginated Membrane in Neurons: Implications for Surface Area RegulationThe Journal of Membrane Biology, 171
J. Heubach, E. Graf, Judith Leutheuser, M. Bock, B. Balana, I. Zahanich, T. Christ, S. Boxberger, E. Wettwer, U. Ravens (2004)
Electrophysiological properties of human mesenchymal stem cellsThe Journal of Physiology, 554
Jonathan Stiber, Zhu-shan Zhang, Jarrett Burch, J. Eu, S. Zhang, G. Truskey, Malini Seth, N. Yamaguchi, G. Meissner, Ripal Shah, P. Worley, R. Williams, P. Rosenberg (2008)
Mice Lacking Homer 1 Exhibit a Skeletal Myopathy Characterized by Abnormal Transient Receptor Potential Channel ActivityMolecular and Cellular Biology, 28
J. Martens, M. Radmacher (2008)
Softening of the actin cytoskeleton by inhibition of myosin IIPflügers Archiv - European Journal of Physiology, 456
A. Kamkin, I. Kiseleva, G. Isenberg (2003)
Ion selectivity of stretch-activated cation currents in mouse ventricular myocytesPflügers Archiv, 446
Amy Collinsworth, S. Zhang, W. Kraus, G. Truskey (2002)
Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation.American journal of physiology. Cell physiology, 283 4
Membrane-cytoskeleton interaction regulates transmembrane currents through stretch-activated channels (SACs); however, the mechanisms involved have not been tested in living cells. We combined atomic force microscopy, confocal immunofluorescence, and patch-clamp analysis to show that stress fibers (SFs) in C2C12 myoblasts behave as cables that, tensed by myosin II motor, activate SACs by modifying the topography and the viscoelastic (Young's modulus and hysteresis) and electrical passive (membrane capacitance, C m ) properties of the cell surface. Stimulation with sphingosine 1-phosphate to elicit SF formation, the inhibition of Rho-dependent SF formation by Y-27632 and of myosin II-driven SF contraction by blebbistatin, showed that not SF polymerization alone but the generation of tensional forces by SF contraction were involved in the stiffness response of the cell surface. Notably, this event was associated with a significant reduction in the amplitude of the cytoskeleton-mediated corrugations in the cell surface topography, suggesting a contribution of SF contraction to plasma membrane stretching. Moreover, C m , used as an index of cell surface area, showed a linear inverse relationship with cell stiffness, indicating participation of the actin cytoskeleton in plasma membrane remodeling and the ability of SF formation to cause internalization of plasma membrane patches to reduce C m and increase membrane tension. SF contraction also increased hysteresis. Together, these data provide the first experimental evidence for a crucial role of SF contraction in SAC activation. The related changes in cell viscosity may prevent SAC from abnormal activation. actin remodeling; atomic force microscopy, Young's modulus; membrane capacitance; hysteresis Address for reprint requests and other correspondence: L. Formigli, Dept. of Anatomy, Histology, Forensic Medicine, Univ. of Florence, Viale Morgagni 85, 50134 Florence, Italy (e-mail: formigli@unifi.it )
AJP - Cell Physiology – The American Physiological Society
Published: Jul 1, 2008
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.